Thread: NMR paper DMSO-Quenched H/D-Exchange 2D NMR Spectroscopy and Its Applications in Protein Science
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Default DMSO-Quenched H/D-Exchange 2D NMR Spectroscopy and Its Applications in Protein Science
DMSO-Quenched H/D-Exchange 2D NMR Spectroscopy and Its Applications in Protein Science

Hydrogen/deuterium (H/D) exchange combined with two-dimensional (2D) NMR spectroscopy has been widely used for studying the structure, stability, and dynamics of proteins. When we apply the H/D-exchange method to investigate non-native states of proteins such as equilibrium and kinetic folding intermediates, H/D-exchange quenching techniques are indispensable, because the exchange reaction is usually too fast to follow by 2D NMR. In this article, we will describe the dimethylsulfoxide...

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