Thread: NMR paper 19 F-NMR shows that active site aromatic residues in CYP121 of Mycobacterial tuberculosisplay a dual role in substrate interaction and protein structure
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Unread 05-14-2022, 03:47 AM
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Default 19 F-NMR shows that active site aromatic residues in CYP121 of Mycobacterial tuberculosisplay a dual role in substrate interaction and protein structure
19 F-NMR shows that active site aromatic residues in CYP121 of Mycobacterial tuberculosisplay a dual role in substrate interaction and protein structure

CYP121 is an essential cytochrome P450 enzyme (CYP) from Mycobacterium tuberculosis.CYP121 mediates an atypical phenol-coupling reaction that converts cyclodityrosine (cYY) into mycocylosin. Our lab has previously employed a ^(19) F nuclear magnetic resonance (NMR) label, 3-bromo-1,1,1-trifluoroacetone (BTFA), to study dynamics of the FG-loop, a region connecting the F and G-helices. In other CYPs, the FG-loop region has been implicated in active site access and substrate recognition. The ^(19)...

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