Biphasic activation of beta-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR
?-arrestins (?arrs) play multifaceted roles in the function of G protein-coupled receptors (GPCRs). ?arrs typically interact with phosphorylated C-terminal tail (C tail) and transmembrane core (TM core) of GPCRs. However, the effects of the C tail- and TM core-mediated interactions on the conformational activation of ?arrs have remained elusive. Here, we show the conformational changes for ?arr activation upon the C tail- and TM core-mediated interactions with a prototypical GPCR by nuclear...
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