Thread: NMR paper Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions
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Default Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions
Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions

Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for mapping and characterizing the binding interface of macromolecular complexes. ¹H-^(15)N-HSQC-based CSP studies have become the method of choice due to their simplicity, short-time requirements, and minimal working knowledge of NMR. CSP studies for characterizing protein-glycosaminoglycan (GAG) interactions can be challenging due to...

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