Protein NMR spectroscopy at 150 kHz magic-angle spinning continues to improve resolution and mass sensitivity.
Related Articles Protein NMR spectroscopy at 150 kHz magic-angle spinning continues to improve resolution and mass sensitivity.
Chembiochem. 2020 Jun 05;:
Authors: Schleedorn M, Malär A, Torosyan A, Penzel S, Klose D, Oss A, Org ML, Wang S, Lecoq L, Cadalbert R, Samoson A, Böckmann A, Meier BH
Abstract
Spectral resolution is key to unleash the structural and dynamic information contained in NMR spectra. Fast magic-angle spinning (MAS) has recently revolutionized the spectroscopy of biomolecular solids. We here report a further remarkable resolution improvement in the spectra of four fully protonated proteins and a small drug molecule, by pushing the MAS rotation frequency even higher, to 150 kHz, than the more routinely used 100 kHz. We observe a reduction of the average homogeneous linewidth by a factor 1.5 and a decrease in the observed linewidth by a factor 1.25. We conclude that even faster MAS is highly attractive and increases mass sensitivity at a moderate price in overall sensitivity.
PMID: 32501630 [PubMed - as supplied by publisher]
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