Advances in instrumentation and methodology for solid-state NMR of biological assemblies.
Related Articles Advances in instrumentation and methodology for solid-state NMR of biological assemblies.
J Struct Biol. 2019 04 01;206(1):73-89
Authors: Martin RW, Kelly JE, Kelz JI
Abstract
Many advances in instrumentation and methodology have furthered the use of solid-state NMR as a technique for determining the structures and studying the dynamics of molecules involved in complex biological assemblies. Solid-state NMR does not require large crystals, has no inherent size limit, and with appropriate isotopic labeling schemes, supports solving one component of a complex assembly at a time. It is complementary to cryo-EM, in that it provides local, atomic-level detail that can be modeled into larger-scale structures. This review focuses on the development of high-field MAS instrumentation and methodology; including probe design, benchmarking strategies, labeling schemes, and experiments that enable the use of quadrupolar nuclei in biomolecular NMR. Current challenges facing solid-state NMR of biological assemblies and new directions in this dynamic research area are also discussed.
PMID: 30205196 [PubMed - indexed for MEDLINE]
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