Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Chem Commun (Camb). 2020 May 19;:
Authors: Habibullah BI, Tripathi V, Surana P, Das R
Abstract
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.
PMID: 32426760 [PubMed - as supplied by publisher]
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