Applications of WaterControl to TOCSY and COSY experiments
Abstract
WaterControl is a solvent suppression method based on WATERGATE and PGSTEÂ*and is very efficient in selectively reducing the solvent signal in 1D pulse-acquire and 2D NOESY of protein solutions. In this study, the WaterControl technique was appended to two common 2D NMR methods used in resonance assignment of proteins, namely TOCSY and CLIP-COSY. Similar to that observed in regular 1D pulse-acquire and 2D NOESY, the incorporation of WaterControl in these 2D methods led to excellent solvent suppression superior to that obtained using W3- or W5-based WATERGATE sequences. The water signal was essentially eliminated in the TOCSY and CLIP-COSY with WaterControl while useful cross peaks around the water resonance at Ï?2 were preserved. This is in contrast to the 2D spectra obtained from the corresponding WATERGATE containing sequences, where these cross peaks in the Ï?2 region are usually suppressed together with the water resonance. These new WaterControl sequences provide significantly improved water suppression thereby facilitating protein NMR studies.
Source: Journal of Biomolecular NMR