Membrane proteins in magnetically aligned phospholipid polymer discs for solid-state NMR spectroscopy.
Related Articles Membrane proteins in magnetically aligned phospholipid polymer discs for solid-state NMR spectroscopy.
Biochim Biophys Acta Biomembr. 2020 May 01;:183333
Authors: Park SH, Wu J, Yao Y, Singh C, Tian Y, Marassi FM, Opella SJ
Abstract
Well-hydrated phospholipid bilayers provide a near-native environment for membrane proteins. They enable the preparation of chemically-defined samples suitable for NMR and other spectroscopic experiments that reveal the structure, dynamics, and functional interactions of the proteins at atomic resolution. The synthetic polymer styrene maleic acid (SMA) can be used to prepare detergent-free samples that form macrodiscs with diameters greater than 30 nm at room temperature, and spontaneously align in the magnetic field of an NMR spectrometer at temperatures above 35 °C. Here we show that magnetically aligned macrodiscs are particularly well suited for solid-state NMR experiments of membrane proteins because the SMA-lipid assembly both immobilizes the embedded protein and provides uniaxial order for oriented sample (OS) solid-state NMR studies. We show that aligned macrodiscs incorporating four different membrane proteins with a wide range of sizes and topological complexity yield high-resolution OS solid-state NMR spectra. The work is dedicated to Michelle Auger who made key contributions to the field of membrane and membrane protein biophysics.
PMID: 32371072 [PubMed - as supplied by publisher]
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