Thread: NMR paper Cost-effective large-scale expression of proteins for NMR studies.
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Default Cost-effective large-scale expression of proteins for NMR studies.
Cost-effective large-scale expression of proteins for NMR studies.

Related Articles Cost-effective large-scale expression of proteins for NMR studies.

J Biomol NMR. 2018 May 19;:

Authors: Klopp J, Winterhalter A, Gébleux R, Scherer-Becker D, Ostermeier C, Gossert AD

Abstract
We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and 13C-methyl methionine labeling in rich media, where yields of up to a gram of soluble protein per liter of culture are reached. Procedures for uniform isotope labeling of 2H, 13C and 15N using auto-induction or isopropyl-?-D-1-thiogalactopyranoside-induction are described, with primary focus on minimal isotope consumption and high reproducibility of protein expression. These protocols are based on high cell-density fermentation, but the key procedures are easily transferred to shake flask cultures.


PMID: 29779067 [PubMed - as supplied by publisher]



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