Solution NMR assignment of the C-terminal domain of human chTOG.
Related Articles Solution NMR assignment of the C-terminal domain of human chTOG.
Biomol NMR Assign. 2018 Mar 26;:
Authors: Rostkova E, Burgess SG, Bayliss R, Pfuhl M
Abstract
The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains-a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of ?-helices. However, the appearance of the 1H-15N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.
PMID: 29582386 [PubMed - as supplied by publisher]
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