High-resolution Solid-state NMR Characterization of Ligand Binding to a Protein Immobilized in a Silica Matrix.
Related Articles High-resolution Solid-state NMR Characterization of Ligand Binding to a Protein Immobilized in a Silica Matrix.
J Phys Chem B. 2017 Aug 01;:
Authors: Cerofolini L, Giuntini S, Louka A, Ravera E, Fragai M, Luchinat C
Abstract
Solid state NMR is becoming a powerful tool to detect atomic-level structural features of biomolecules even when they are bound to - or trapped in - solid systems that lack long-range three dimensional order. We here demonstrate that it is possible to probe protein-ligand interactions from a protein-based perspective also when the protein is entrapped in silica, thus translating into biomolecular solid state NMR all the considerations that are usually made to understand the chemical nature of the interaction of a protein with its ligands. This work provides the proof of concept that also immobilized enzymes can be used for protein-based NMR protein-ligand interactions for drug discovery.
PMID: 28762736 [PubMed - as supplied by publisher]
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