The Potential of Polygonum cuspidatum Root as an Antidiabetic Food - Dual High-Resolution ?-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents.
Related Articles The Potential of Polygonum cuspidatum Root as an Antidiabetic Food - Dual High-Resolution ?-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents.
J Agric Food Chem. 2017 May 12;:
Authors: Zhao Y, Chen MX, Kongstad KT, Jager AK, Staerk D
Abstract
The Worldwide increasing incidence of type 2 diabetes has fuelled an intensified search for food and herbal remedies with preventive and/or therapeutic properties. Polygonum cuspidatum Siebold & Zucc. (Polygonaceae) is used as a functional food in Japan and South Korea, and it's also a well-known traditional antidiabetic herb used in China. In this study, dual high-resolution ?-glucosidase and protein-tyrosine phosphatase 1B (PTP1B) inhibition profiling was used for identification of individual antidiabetic constituents directly from the crude ethyl acetate extract and fractions of P. cuspidatum. Subsequent preparative-scale HPLC was used to isolate a series of ?-glucosidase inhibitors, which after HPLC-HRMS and NMR analysis were identified as procyanidin B2 3,3''-O-di-gallate (3) and (-)-epicatechin gallate (5) with IC50 values of 0.42 ± 0.02 and 0.48 ± 0.0004 ?M, respectively, as well as a series of stilbene analogues with IC50 value in the range from 6.05 ± 0.05 ?M to 116.10 ± 2.04 ?M. In addition, (trans)-emodin-physcion bianthrone (15b) and (cis)-emodin-physcion bianthrone (15c) were identified as potent PTP1B inhibitors with IC50 values of 2.77 ± 1.23 and 7.29 ± 2.32 ?M, respectively. These findings show that P. cuspidatum is a potential functional food for management of type 2 diabetes.
PMID: 28497962 [PubMed - as supplied by publisher]
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