Thread: NMR paper Algal autolysate medium to label proteins for NMR in mammalian cells.
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Default Algal autolysate medium to label proteins for NMR in mammalian cells.
Algal autolysate medium to label proteins for NMR in mammalian cells.

Related Articles Algal autolysate medium to label proteins for NMR in mammalian cells.

J Biomol NMR. 2016 Apr 22;

Authors: Fuccio C, Luchinat E, Barbieri L, Neri S, Fragai M

Abstract
In-cell NMR provides structural and functional information on proteins directly inside living cells. At present, the high costs of the labeled media for mammalian cells represent a limiting factor for the development of this methodology. Here we report a protocol to prepare a homemade growth medium from Spirulina platensis autolysate, suitable to express uniformly labeled proteins inside mammalian cells at a reduced cost-per-sample. The human proteins SOD1 and Mia40 were overexpressed in human cells grown in (15)N-enriched S. platensis algal-derived medium, and high quality in-cell NMR spectra were obtained.


PMID: 27106902 [PubMed - as supplied by publisher]



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