A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time.
Related Articles A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time.
J Am Chem Soc. 2015 May 27;137(20):6468-71
Authors: Thongwichian R, Kosten J, Benary U, Rose HM, Stuiver M, Theillet FX, Dose A, Koch B, Yokoyama H, Schwarzer D, Wolf J, Selenko P
Abstract
Cell signaling is governed by dynamic changes in kinase and phosphatase activities, which are difficult to assess with discontinuous readout methods. Here, we introduce an NMR-based reporter approach to directly identify active kinases and phosphatases in complex physiological environments such as cell lysates and to measure their individual activities in a semicontinuous fashion. Multiplexed NMR profiling of reporter phosphorylation states provides unique advantages for kinase inhibitor studies and reveals reversible modulations of cellular enzyme activities under different metabolic conditions.
PMID: 25963544 [PubMed - indexed for MEDLINE]
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