19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy
Abstract
In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted chemical shift dispersion in only four different nucleotides spectral resolution remains limited in larger RNAs. Labeling RNAs with the NMR-active nucleus 19F has previously been introduced for small RNAs up to 40 nucleotides (nt). In the presented work, we study the natural occurring RNA aptamer domain of the guanine-sensing riboswitch comprising 73 nucleotides from
Bacillus subtilis. The work includes protocols for improved in vitro transcription of 2-fluoroadenosine-5â?²-triphosphat (2F-ATP) using the mutant P266L of the T7 RNA polymerase. Our NMR analysis shows that the secondary and tertiary structure of the riboswitch is fully maintained and that the specific binding of the cognate ligand hypoxanthine is not impaired by the introduction of the 19F isotope. The thermal stability of the 19F-labeled riboswitch is not altered compared to the unmodified sequence, but local base pair stabilities, as measured by hydrogen exchange experiments, are modulated. The characteristic change in the chemical shift of the imino resonances detected in a 1H,15N-HSQC allow the identification of Watsonâ??Crick base paired uridine signals and the 19F resonances can be used as reporters for tertiary and secondary structure transitions, confirming the potential of 19F-labeling even for sizeable RNAs in the range of 70 nucleotides.
Source: Journal of Biomolecular NMR