Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Curr Opin Struct Biol. 2015 Feb 26;32C:39-47
Authors: Smith MJ, Marshall CB, Theillet FX, Binolfi A, Selenko P, Ikura M
Abstract
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.
PMID: 25727665 [PubMed - as supplied by publisher]
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