Unlike twins: an NMR comparison of two ?-synuclein polymorphs featuring different toxicity.
Related Articles Unlike twins: an NMR comparison of two ?-synuclein polymorphs featuring different toxicity.
PLoS One. 2014;9(3):e90659
Authors: Gath J, Bousset L, Habenstein B, Melki R, Böckmann A, Meier BH
Abstract
We structurally compare, using solid-state NMR, two different polymorphs of ?-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While ?-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified ?-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for
sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results.
PMID: 24599158 [PubMed - indexed for MEDLINE]
More...