Thread: Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS
View Single Post
  #1  
Unread 10-29-2014, 03:51 PM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,204
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS
From The DNP-NMR Blog:

Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS


Friesen-Waldner, L.J., et al., Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS. Chem Commun (Camb), 2014. 50(89): p. 13801-4.


http://www.ncbi.nlm.nih.gov/pubmed/25253534


A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 muM were detected at a temporal resolution of 1 s.


Go to The DNP-NMR Blog for more info.
Reply With Quote

Did you find this post helpful? Yes | No