Magnetic susceptibility to measure total protein concentration from NMR metabolite spectra: Demonstration on blood plasma.
Related Articles Magnetic susceptibility to measure total protein concentration from NMR metabolite spectra: Demonstration on blood plasma.
Magn Reson Med. 2014 Mar 17;
Authors: Jupin M, Michiels PJ, Girard FC, Wijmenga SS
Abstract
PURPOSE: Accurate metabolite and protein quantification in blood plasma and other body fluids from one single NMR measurement, allowing for improved quantitative metabolic profiling and better assessment of metabolite-protein interactions.
THEORY AND METHODS: The total protein concentration is derived from the common chemical-shift changes-caused by protein-induced bulk magnetic susceptibility (BMS)-measured on well-accessible and exchange-free metabolite resonances. These BMS shifts are simply obtained by external referencing with respect to 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, sodium salt in a coaxial insert.
RESULTS: Based on blood-plasma data from five volunteers, the estimated accuracy of the BMS method is 10 g/L). Also, it allows measurement of the presence and sign of exchange-induced chemical-shift changes. Magn Reson Med, 2014. © 2014 Wiley Periodicals, Inc.
PMID: 24639074 [PubMed - as supplied by publisher]
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