Thread: Help!!Why does a deuterated protein behave even more poorly than the protonated one?
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Unread 11-13-2006, 01:35 PM
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Default Help!!Why does a deuterated protein behave even more poorly than the protonated one?
A 20kDa protein, about half of the total signals can be observed in CBCA(CO)NH experiment when protonated with sample concentration of 1mM. However, after deuterated, hardly any signals can be observed with the same concentration in this experiment. We use the same pulse sequence except adding deuterium decoupling in the deuterated one. I don't know why? Help!!
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