Thread: Question from NMRWiki Q&A forum How to get off resonance ligand integral in STD experiment
View Single Post
  #1  
Unread 06-27-2013, 01:52 AM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,199
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default How to get off resonance ligand integral in STD experiment
How to get off resonance ligand integral in STD experiment

Hello I am using satxfer1D experiment on varian unity plus 500 MHz spectrometer and vnmr (not vnmrJ!) software. I would like to determine dissociation constant from STD amplification factor (Io-Isat)/Io. So I need separate spectrum saturated at 30ppm to get off-resonance ligand integrals Io. Standard STD experiment give me only the difference between on- and off- resonance spectra (Io-Isat). Is the possibility to extract or to programm satxfer1D experiment to get what I want?



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote

Did you find this post helpful? Yes | No