Current applications of 19F NMR to studies of protein structure and dynamics
April 2012
Publication year: 2012
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 62
Highlights
? 19F molecular tags and labeling protocols for 19F NMR studies of proteins are reviewed and contrasted. ? 19F NMR biosynthetic labeling strategies are presented. ? Experimental challenges (loss of function through labeling, line broadening, assignment ambiguities) are discussed. ? Approaches to the study of protein topology, using 19F NMR, are presented. ? Current examples of protein NMR studies are given.
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