Covariance spectroscopy with a non-uniform and consecutive acquisition scheme for signal enhancement of the NMR experiments
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 6 March 2012
Yixuan*Li, Qiang*Wang, Zhengfeng*Zhang, Jun*Yang, Bingwen*Hu, ...
Two-dimensional covariance (COV2D) spectroscopy with non-uniform and consecutive acquisition (NUCA) scheme is introduced. This NUCA-COV2D method allows the number of t1points to be reduced by a factor of 1.5~3 without any broadening of the linewidth. Furthermore, the signal-to-noise ratio (S/N) can be increased up to 50%, which can further save experimental time by another factor of 2. This method has been demonstrated with model samples and the microcrystalline proteins. In all cases, the total experimental time can be reduced by a factor of 3~6, without any loss of resolution and S/N, with respect to what is necessary with the FT2D NMR.
Graphical abstract
Highlights
? Compared with UCA-FT2D, NUCA-COV2D achieves better resolution and saves considerable experimental time. ? Compared with NUCA-FT2D, NUCA-COV2D achieves better S/N and resolution and saves considerable experimental time. ? Compared with UCA-COV2D, NUCA-COV2D increases the signal-to-noise ratio (S/N) by a factor of 2.
Source:
Journal of Magnetic Resonance