Thread: Question from NMRWiki Q&A forum 3d Tocsy-hsqc mgnetization transfer problem
View Single Post
  #1  
Unread 03-06-2012, 06:17 PM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,191
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default 3d Tocsy-hsqc mgnetization transfer problem
3d Tocsy-hsqc mgnetization transfer problem

Dear All,I acquired a 3d tocsy-hsqc edited on Bruker machine of small protein in micelles with a mixing time of 70ms. The problem is that I do not observe almoust spin system of each aminoacid, in detail I see only cross peaks between NH-HA. I obtained very good results by 3d Noesy-hsqc edited. How can I do to improve the magnetization transfer along the spin system and see all cross peaks? Do you think that this problem is due to the nature?Thank you in advance\



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote

Did you find this post helpful? Yes | No