Thread: Question from NMRWiki Q&A forum RDC gel preparation
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Unread 01-24-2012, 07:54 PM
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Default RDC gel preparation
RDC gel preparation

I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the following 1)drying the gel and re-swelling with protein solution2)allowing equilibration of protein into an already swelled gel

It seems as though you can get a much higher protein conc. if you dry the gel so what is the advantage to letting it equilibrate?

Thanks



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