Thread: Question from NMRWiki Q&A forum Randomly duterated protein is useful to get noesy experments ?
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Unread 10-03-2011, 08:39 AM
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Default Randomly duterated protein is useful to get noesy experments ?
Randomly duterated protein is useful to get noesy experments ?

Dear NMR WIKIers

I got doubt that can I use Randomly duterated protein (40 to 60 percent ) to get 3D (C13 EDIT and N15 EDIT) noesy experiments ? because if i duteriated the protein randomly the peak intensity drop would be there. but from standard formula NOE = 1/R6.
I am afraid of error from intensity drop .

could you please suggest any reference related to this ?

Thnaking you



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