1H Assisted 13C/15N Heteronuclear Correlation Spectroscopy in Oriented Sample Solid-State NMR of Single Crystal and Magnetically Aligned Samples
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 9 April 2011
Eugene C., Lin , Stanley J., Opella
1H-irradiation under mismatched Hartmann-Hahn conditions provides an alternative mechanism for carrying out 15N/13C transfers in triple-resonance heteronuclear correlation spectroscopy (HETCOR) on stationary samples of single crystals and aligned samples of biopolymers, which improve the efficiency especially when the direct 15N-13C dipolar couplings are small. In many cases, the sensitivity is improved by taking advantage of the 13C? labeled sites in peptides and proteins with 13C detection. The similarities between experimental and simulated spectra demonstrate the validity of the recoupling mechanism and identify the potential for applying these experiments to virus particles or membrane proteins in phospholipid bilayers; however, further development...
Graphical abstract
*Graphical abstract:**Highlights:*? Heteronuclear dipolar couplings differ by two in perpendicular and parallel bilayers ? Dipolar couplings correlate resonances in perpendicular and parallel bilayers ? Assignments result from the dipolar correlation and an assigned isotropic spectrum
Source:
Journal of Magnetic Resonance