Solution NMR Spectroscopy of Supra-Molecular Systems, Why Bother? A Methyl TROSY View
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 8 March 2011
Lewis E., Kay
With the development of appropriate labeling schemes and the associated experiments that exploit them it has become possible to record high quality solution NMR spectra of supra-molecular complexes with molecular masses extending to 1MDa. One such approach involves selective 13CH3 methyl labeling in highly deuterated proteins using experiments that make use of a methyl-TROSY effect that significantly improves both resolution and sensitivity in spectra. The utility of this methodology has been demonstrated on a growing number of interesting particles. It seems appropriate at this juncture, therefore, to ‘step back’ and evaluate the role that solution NMR spectroscopy can play in...
Graphical abstract
*Graphical abstract:*Studies of MDa complexes by solution-state NMR using Methyl-TROSY: An application to the proteasome.*Highlights:*? Qualitative explanation of how 1H-13C and 1H-1H dipolar cross-correlated relaxation in a 13CH3 group attached to a macromolecule can be exploited for studies of supra-molecular structure, dynamics and function. Studies of the proteasome (670 kDa) via methyl-TROSY NMR emphasize the importance of dynamics to the function of this large complex. Studies of proteasome substrates show that they are maintained in a disordered state inside the antechamber.
Source:
Journal of Magnetic Resonance