NMR spectroscopy tools for structure-aided drug design.
Related Articles NMR spectroscopy tools for structure-aided drug design.
Angew Chem Int Ed Engl. 2004 Jan 3;43(3):290-300
Authors: Homans SW
Biomolecular NMR spectroscopy has expanded dramatically in recent years and is now a powerful tool for the study of structure, dynamics, and interactions of biomolecules. Previous limitations with respect to molecular size are no longer a primary barrier, and systems as large as 900 kDa were recently studied. NMR spectroscopy is already well-established as an efficient method for ligand screening. A number of recently developed techniques show promise as aids in structure-based drug design, for example, in the rapid determination of global protein folds, the structural characterization of ligand-protein complexes, and the derivation of thermodynamic parameters. An advantage of the method is that all these interactions can be studied in solution--time-consuming crystallization is not necessary. This Review focuses on recent developments in NMR spectroscopy and how they might be of value in removing some of the current "bottlenecks" in structure-based drug discovery.
PMID: 14705081 [PubMed - indexed for MEDLINE]
Source:
PubMed