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Investigation of lipid/protein interactions in trifluoroethanol-water mixtures proposes the strategy for the refolding of helical transmembrane domains
Dec 01, 2022 - 2:25 PM - by nmrlearner
nmrlearner's Avatar Investigation of lipid/protein interactions in trifluoroethanol-water mixtures proposes the strategy for the refolding of helical transmembrane domains

Abstract

Membrane proteins are one of the keystone objects in molecular biology, but their structural studies often require an extensive search for an appropriate membrane-like environment and an efficient refolding protocol for a recombinant protein. Isotropic bicelles are a convenient membrane mimetic used in structural studies of membrane proteins. Helical membrane domains are often transferred into bicelles from trifluoroethanolâ??water mixtures. However, the protocols for such a refolding are empirical and the process itself is still not understood in detail. In search of the optimal refolding approaches for helical membrane proteins, we studied here how membrane proteins, lipids, and detergents interact with each other at various trifluoroethanolâ??water ratios. Using high-resolution NMR spectroscopy and dynamic light scattering, we determined the key states of the listed compounds in the trifluoroethanol/water mixture, found the factors that could be critical for the efficiency of refolding, and proposed several most optimal protocols. These protocols were developed on the transmembrane domain of neurotrophin receptor TrkA and tested on two model helical membrane domainsâ??transmembrane of Toll-like receptor TLR9 and voltage-sensing domain of a potassium channel KvAP.



Source: Journal of Biomolecular NMR
0 Replies | 10 Views
Moderate alcohol intake may affect lipoprotein metabolism - News-Medical.Net
Dec 01, 2022 - 2:25 PM - by nmrlearner
nmrlearner's Avatar Moderate alcohol intake may affect lipoprotein metabolism - News-Medical.Net

Moderate alcohol intake may affect lipoprotein metabolism News-Medical.Net Read here
0 Replies | 7 Views
Scientists uncovered the structure of the key protein for a future hepatitis C vaccine – here's how they did it - The Conversation
Dec 01, 2022 - 2:25 PM - by nmrlearner
nmrlearner's Avatar Scientists uncovered the structure of the key protein for a future hepatitis C vaccine – here's how they did it The Conversation
Scientists uncovered the structure of the key protein for a future hepatitis C vaccine – here's how they did it - The Conversation
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0 Replies | 6 Views
[NMR paper] Metabolic profiling of colorectal cancer organoids: a comparison between HR MAS MRS and solution NMR spectroscopy of polar extracts
Dec 01, 2022 - 2:25 PM - by nmrlearner
nmrlearner's Avatar Metabolic profiling of colorectal cancer organoids: a comparison between HR MAS MRS and solution NMR spectroscopy of polar extracts

Patient-derived cancer cells cultured in vitro is a cornerstone for cancer metabolism research. More recently, the introduction of organoids has provided the research community with a more versatile model system. Physiological structure and organization of the cell source tissue is maintained in organoids, representing a closer link to in vivo tumor models. High resolution magic angle spinning MR spectroscopy (HR MAS MRS) is a commonly applied analytical approach for metabolic profiling of...

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0 Replies | 9 Views
NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems - Phys.org
Dec 01, 2022 - 1:32 AM - by nmrlearner
nmrlearner's Avatar NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems - Phys.org

NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems Phys.org Read here
0 Replies | 13 Views
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