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[ASAP] Pressure-Sensitive and Osmolyte-Modulated Liquid–Liquid Phase Separation of Eye-Lens ?-Crystallins
Apr 24, 2019 - 9:00 AM - by nmrlearner
nmrlearner's Avatar [ASAP] Pressure-Sensitive and Osmolyte-Modulated Liquid–Liquid Phase Separation of Eye-Lens ?-Crystallins

Süleyman Cinar, Hasan Cinar, Hue Sun Chan, Roland Winter



Journal of the American Chemical Society
DOI: 10.1021/jacs.8b13636




Source: Journal of the American Chemical Society
0 Replies | 5 Views
Global Protein Crystallography Market Trend 2019 – Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher - Industry News Daily
Apr 24, 2019 - 9:00 AM - by nmrlearner
nmrlearner's Avatar Global Protein Crystallography Market Trend 2019 – Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher - Industry News Daily

Global Protein Crystallography Market Trend 2019 – Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher Industry News DailyThe Global Protein Crystallography Market Report study with in-depth overview, describing the Product/Industry Scope and elaborates market outlook and status ...

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0 Replies | 3 Views
Identification of the substrate recruitment mechanism of the muscle glycogen protein phosphatase 1 holoenzyme - Science Advances
Apr 24, 2019 - 9:00 AM - by nmrlearner
nmrlearner's Avatar Identification of the substrate recruitment mechanism of the muscle glycogen protein phosphatase 1 holoenzyme - Science Advances

Identification of the substrate recruitment mechanism of the muscle glycogen protein phosphatase 1 holoenzyme Science AdvancesGlycogen is the primary storage form of glucose. Glycogen synthesis and breakdown are tightly controlled by glycogen synthase (GYS) and phosphorylase, ...

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0 Replies | 5 Views
Fatigue in multiple sclerosis: Protein biomarkers - Ezine - spectroscopyNOW.com
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Fatigue in multiple sclerosis: Protein biomarkers - Ezine - spectroscopyNOW.com

Fatigue in multiple sclerosis: Protein biomarkers - Ezine spectroscopyNOW.comMultiple sclerosis and fatigue Fatigue is one of the most debilitating symptoms of multiple sclerosis, rendering sufferers incapable of any activity in its most ...

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0 Replies | 11 Views
Photocycle-dependent conformational changes in the proteorhodopsin cross-protomer Asp-His-Trp triad revealed by DNP-enhanced MAS-NMR [Biophysics and Computational Biology]
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Photocycle-dependent conformational changes in the proteorhodopsin cross-protomer Asp-His-Trp triad revealed by DNP-enhanced MAS-NMR [Biophysics and Computational Biology]

Jakob Maciejko, Jagdeep Kaur, Johanna Becker-Baldus, Clemens Glaubitz...
Date: 2019-04-23

Proteorhodopsin (PR) is a highly abundant, pentameric, light-driven proton pump. Proton transfer is linked to a canonical photocycle typical for microbial ion pumps. Although the PR monomer is able to undergo a full photocycle, the question arises whether the pentameric complex formed in the membrane via specific cross-protomer interactions plays... Read More


PNAS:
Number: 17
Volume: 116
0 Replies | 11 Views
[NMR paper] pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.

Related Articles pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.

Proc Natl Acad Sci U S A. 2018 11 27;115(48):12194-12199

Authors: Otieno SA, Hanz SZ, Chakravorty B, Zhang A, Klees LM, An M, Qiang W


Abstract
The applications of the pH low insertion peptide (pHLIP) in cancer diagnosis and cross-membrane cargo delivery have drawn increasing attention in the past decade. With its origin as the transmembrane (TM) helix C of bacteriorhodopsin, pHLIP is also an important model for understanding how pH can affect the folding and topogenesis of a TM ?-helix. Protonations of multiple D/E residues transform pHLIP from an unstructured coil at membrane surface (known as state II, at pH >= 7) to a TM ?-helix (state III, pH
0 Replies | 10 Views
[NMR paper] Stability of Nitroxide Biradical TOTAPOL in Biological Samples
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Stability of Nitroxide Biradical TOTAPOL in Biological Samples

Publication date: Available online 22 April 2019

Source: Journal of Magnetic Resonance

Author(s): Kelsey McCoy, Rivkah Rogawski, Olivia Stovicek, Ann McDermott

Abstract

We characterize chemical reduction of a nitroxide biradical, TOTAPOL, used in dynamic nuclear polarization (DNP) experiments, specifically probing the stability in whole-cell pellets and lysates, and present a few strategies to stabilize the biradicals for DNP studies. DNP solid-state NMR experiments use paramagnetic species such as nitroxide biradicals to dramatically increase NMR signals. Although there is considerable excitement about using nitroxide- based DNP for detecting the NMR spectra of proteins in whole cells, nitroxide radicals are reduced in minutes in bacterial cell pellets, which we confirm and quantify here. We show that addition of the covalent cysteine blocker N-ethylmaleimide to whole cells significantly slows the rate of reduction, suggesting that cysteine thiol radicals are important to in vivo radical reduction. The use of cell lysates rather than whole cells also slows TOTAPOL reduction, which suggests a possible role for the periplasm and oxidative phosphorylation metabolites in radical degradation. Reduced TOTAPOL in lysates can also be efficiently reoxidized with potassium ferricyanide. These results point to a practical and robust set of strategies for DNP of cellular preparations.



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