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NMR Provides Unique Insight into the Functional Dynamics and Interactions of Intrinsically Disordered Proteins - ACS Publications
Mar 23, 2025 - 9:41 PM - by nmrlearner
nmrlearner's Avatar NMR Provides Unique Insight into the Functional Dynamics and Interactions of Intrinsically Disordered Proteins - ACS Publications

NMR Provides Unique Insight into the Functional Dynamics and Interactions of Intrinsically Disordered Proteins ACS Publications Read here
0 Replies | 13 Views
[NMR paper] Solid-state NMR observation of chitin in whole cells by indirect (15)N detection with NC, NCC, CNC and CNCC polarization transfers
Mar 23, 2025 - 9:41 PM - by nmrlearner
nmrlearner's Avatar Solid-state NMR observation of chitin in whole cells by indirect (15)N detection with NC, NCC, CNC and CNCC polarization transfers

Chitin is the most important nitrogen-containing polysaccharide found on Earth. This polysaccharide is a polymer of an N-acetylglucosamine and it is a crucial structural component of fungal cell walls and crustaceans. Magic-angle spinning solid-state NMR is emerging as a powerful analytical approach to study polysaccharides in the context of intact cell walls and whole cells. The presence of an acetamido group in chitin is attractive for ^(15)N solid-state NMR. Here we investigate the use of...

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0 Replies | 25 Views
[NMR paper] In situ light-driven pH modulation for NMR studies
Mar 23, 2025 - 9:41 PM - by nmrlearner
nmrlearner's Avatar In situ light-driven pH modulation for NMR studies

Proton exchange is a fundamental chemical event, and NMR provides the most direct readout of protonation events with site-specific resolution. Conventional approaches require manual titration of sample pH to collect a series of NMR spectra at different pH values. This requires extensive sample handling and often results in significant sample loss, leading to reduced signal or the need to prepare additional samples. Here, we introduce a novel approach to control pH in NMR samples using water...

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0 Replies | 11 Views
Protein 19F NMR in Escherichia coli - ACS Publications
Mar 23, 2025 - 9:18 AM - by nmrlearner
nmrlearner's Avatar Protein 19F NMR in Escherichia coli - ACS Publications

Protein 19F NMR in Escherichia coli ACS Publications Read here
0 Replies | 10 Views
Exploring Protein Structures by DNP-Enhanced Methyl Solid-State NMR Spectroscopy - ACS Publications
Mar 23, 2025 - 9:18 AM - by nmrlearner
nmrlearner's Avatar Exploring Protein Structures by DNP-Enhanced Methyl Solid-State NMR Spectroscopy - ACS Publications

Exploring Protein Structures by DNP-Enhanced Methyl Solid-State NMR Spectroscopy ACS Publications Read here
0 Replies | 12 Views
[NMR paper] Solid-State NMR of Membrane Peptides and Proteins in the Lipid Cubic Phase
Mar 23, 2025 - 9:18 AM - by nmrlearner
nmrlearner's Avatar Solid-State NMR of Membrane Peptides and Proteins in the Lipid Cubic Phase

Solid-state nuclear magnetic resonance (ssNMR) is a powerful technique for studying membrane protein structure and dynamics. Ideally, measurements are performed with the protein in a lipid bilayer. However, homogenous reconstitution of functional protein into intact bilayers at sufficiently high concentrations is often difficult to achieve. In this work, we investigate the suitability of the lipid cubic phase (LCP), which incorporates a lipid bilayer, as an alternative medium for ssNMR of...

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0 Replies | 44 Views
[NMR paper] In situ light-driven pH modulation for NMR studies
Mar 23, 2025 - 9:18 AM - by nmrlearner
nmrlearner's Avatar In situ light-driven pH modulation for NMR studies


Angewandte Chemie International Edition, Accepted Article.

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0 Replies | 23 Views
Estimating Protein-Ligand Binding Affinity Using High-Throughput Screening by NMR - ACS Publications
Mar 22, 2025 - 9:12 PM - by nmrlearner
nmrlearner's Avatar Estimating Protein-Ligand Binding Affinity Using High-Throughput Screening by NMR - ACS Publications

Estimating Protein-Ligand Binding Affinity Using High-Throughput Screening by NMR ACS Publications Read here
0 Replies | 14 Views
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