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 Global Protein Crystallography Market Trend 2019 – Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher - Industry News Daily |
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Apr 24, 2019 - 9:00 AM - by nmrlearner
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Global Protein Crystallography Market Trend 2019 – Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher - Industry News Daily
Global Protein Crystallography Market Trend 2019 – Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher Industry News DailyThe Global Protein Crystallography Market Report study with in-depth overview, describing the Product/Industry Scope and elaborates market outlook and status ...
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[NMR paper] pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy. |
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Apr 23, 2019 - 7:54 PM - by nmrlearner
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pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.
 Related Articles pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.
Proc Natl Acad Sci U S A. 2018 11 27;115(48):12194-12199
Authors: Otieno SA, Hanz SZ, Chakravorty B, Zhang A, Klees LM, An M, Qiang W
Abstract
The applications of the pH low insertion peptide (pHLIP) in cancer diagnosis and cross-membrane cargo delivery have drawn increasing attention in the past decade. With its origin as the transmembrane (TM) helix C of bacteriorhodopsin, pHLIP is also an important model for understanding how pH can affect the folding and topogenesis of a TM ?-helix. Protonations of multiple D/E residues transform pHLIP from an unstructured coil at membrane surface (known as state II, at pH >= 7) to a TM ?-helix (state III, pH
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[NMR paper] Stability of Nitroxide Biradical TOTAPOL in Biological Samples |
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Apr 23, 2019 - 7:54 PM - by nmrlearner
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Stability of Nitroxide Biradical TOTAPOL in Biological Samples
Publication date: Available online 22 April 2019
Source: Journal of Magnetic Resonance
Author(s): Kelsey McCoy, Rivkah Rogawski, Olivia Stovicek, Ann McDermott
Abstract
We characterize chemical reduction of a nitroxide biradical, TOTAPOL, used in dynamic nuclear polarization (DNP) experiments, specifically probing the stability in whole-cell pellets and lysates, and present a few strategies to stabilize the biradicals for DNP studies. DNP solid-state NMR experiments use paramagnetic species such as nitroxide biradicals to dramatically increase NMR signals. Although there is considerable excitement about using nitroxide- based DNP for detecting the NMR spectra of proteins in whole cells, nitroxide radicals are reduced in minutes in bacterial cell pellets, which we confirm and quantify here. We show that addition of the covalent cysteine blocker N-ethylmaleimide to whole cells significantly slows the rate of reduction, suggesting that cysteine thiol radicals are important to in vivo radical reduction. The use of cell lysates rather than whole cells also slows TOTAPOL reduction, which suggests a possible role for the periplasm and oxidative phosphorylation metabolites in radical degradation. Reduced TOTAPOL in lysates can also be efficiently reoxidized with potassium ferricyanide. These results point to a practical and robust set of strategies for DNP of cellular preparations.
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