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Direct Crosstalk Between O-GlcNAcylation and Phosphorylation of Tau Protein Investigated by NMR Spectroscopy - Frontiers
Dec 04, 2024 - 12:40 AM - by nmrlearner
nmrlearner's Avatar Direct Crosstalk Between O-GlcNAcylation and Phosphorylation of Tau Protein Investigated by NMR Spectroscopy - Frontiers

Direct Crosstalk Between O-GlcNAcylation and Phosphorylation of Tau Protein Investigated by NMR Spectroscopy Frontiers Read here
0 Replies | 9 Views
[NMR paper] Expanding the Toolset of Biomolecular NMR with Efficient and Cost-Effective 17O-Labeling via Bacterial Expression
Dec 02, 2024 - 3:01 PM - by nmrlearner
nmrlearner's Avatar Expanding the Toolset of Biomolecular NMR with Efficient and Cost-Effective 17O-Labeling via Bacterial Expression

Oxygen plays a central role in biomolecular structures and functions, with 17O NMR emerging as a powerful tool for elucidating biomolecular properties. However, the low natural abundance of the NMR-active isotope, 17O (0.0373%), presents a significant hurdle to its widespread application. Here, we introduce a rapid and cost-effective approach for amino acid-specific 17O-labeling of recombinant proteins. Using a common bacterial expression system and with a 30-minute rapid synthesis protocol of...

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0 Replies | 82 Views
Simultaneous Assignment and Structure Determination of Proteins From Sparsely Labeled NMR Datasets - Frontiers
Nov 29, 2024 - 11:29 PM - by nmrlearner
nmrlearner's Avatar Simultaneous Assignment and Structure Determination of Proteins From Sparsely Labeled NMR Datasets - Frontiers

Simultaneous Assignment and Structure Determination of Proteins From Sparsely Labeled NMR Datasets Frontiers Read here
0 Replies | 39 Views
[NMR paper] (1)H, (13)C, and (15)N resonance assignments of the amyloidogenic peptide SEM2(49-107) by NMR spectroscopy
Nov 29, 2024 - 11:29 PM - by nmrlearner
nmrlearner's Avatar (1)H, (13)C, and (15)N resonance assignments of the amyloidogenic peptide SEM2(49-107) by NMR spectroscopy

It has been shown that human seminal fluid is a major factor in enhancing HIV activity. The SEM2(49-107) peptide is a product of cleavage after ejaculation by internal prostheses of the semenogelin 2 protein, expressed in seminal vesicles. It is established that the peptide SEM2(49-107) forms amyloid fibrils, which increase probability of contracting HIV infection. In this nuclear magnetic resonance (NMR) study, we present almost complete (86%) resonance assignments for the ¹H ^(15)N and ^(13)C...

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0 Replies | 221 Views
[NMR paper] (199)Hg NMR Shielding and Chemical Shifts of 2-, 3-, and 4-Coordinate Hg(II)-Thiolate Species
Nov 29, 2024 - 11:29 PM - by nmrlearner
nmrlearner's Avatar (199)Hg NMR Shielding and Chemical Shifts of 2-, 3-, and 4-Coordinate Hg(II)-Thiolate Species

Spectroscopic characterization of Hg(II)-thiolate complexes is central to the bioinorganic chemistry of Hg(II). Interpretation of ^(199)Hg NMR usually relies on data recorded for reference compounds; however, here, it is demonstrated that ^(199)Hg NMR chemical shifts may be calculated within 40 ppm of experimental values for 2-, 3-, and 4-coordinate Hg(II)-thiolate complexes, using the PBE0 functional, the QZ4P basis set on Hg and S and TZP on all other atoms, and SO-ZORA to account for...

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0 Replies | 31 Views
An Introduction to Protein Purification: Methods, Technologies and Applications - Technology Networks
Nov 29, 2024 - 11:06 AM - by nmrlearner
nmrlearner's Avatar An Introduction to Protein Purification: Methods, Technologies and Applications - Technology Networks

An Introduction to Protein Purification: Methods, Technologies and Applications Technology Networks Read here
0 Replies | 53 Views
NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression - Science
Nov 28, 2024 - 5:11 PM - by nmrlearner
nmrlearner's Avatar NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression - Science

NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression Science Read here
0 Replies | 49 Views
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