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Paramagnetic NMR spectroscopy: getting to the heart of metalloproteins - Wiley
Sep 16, 2024 - 11:19 AM - by nmrlearner
nmrlearner's Avatar Paramagnetic NMR spectroscopy: getting to the heart of metalloproteins - Wiley

Paramagnetic NMR spectroscopy: getting to the heart of metalloproteins Wiley Read here
0 Replies | 6 Views
Cryo-EM and solid state NMR together provide a more comprehensive structural investigation of protein fibrils
Sep 15, 2024 - 8:26 AM - by nmrlearner
nmrlearner's Avatar Cryo-EM and solid state NMR together provide a more comprehensive structural investigation of protein fibrils

Abstract

The tropomyosin 1 isoform I/C C-terminal domain (Tm1-LC) fibril structure is studied jointly with cryogenic electron microscopy (cryo-EM) and solid state nuclear magnetic resonance (NMR). This study demonstrates the complementary nature of these two structural biology techniques. Chemical shift assignments from solid state NMR are used to determine the secondary structure at the level of individual amino acids, which is faithfully seen in cryo-EM reconstructions. Additionally, solid state NMR demonstrates that the region not observed in the reconstructed cryo-EM density is primarily in a highly mobile random coil conformation rather than adopting multiple rigid conformations. Overall, this study illustrates the benefit of investigations combining cryo-EM and solid state NMR to investigate protein fibril structure.



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0 Replies | 117 Views
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.org
Sep 13, 2024 - 3:28 AM - by nmrlearner
nmrlearner's Avatar Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.org

Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale Phys.org Read here
0 Replies | 38 Views
Time-resolved NMR detection of prolyl-hydroxylation in intrinsically disordered region of HIF-1?
Sep 12, 2024 - 11:15 AM - by nmrlearner
nmrlearner's Avatar Time-resolved NMR detection of prolyl-hydroxylation in intrinsically disordered region of HIF-1?

Wenguang HeGeneviève M. C. Gasmi-SeabrookMitsuhiko IkuraJeffrey E. LeeMichael OhhaDepartment of Biochemistry, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5G 1M1, CanadabPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1L7, CanadacDepartment of Medical Biophysics, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5G 1L7, CanadadDepartment of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada...
Date:

Proceedings of the National Academy of Sciences, Volume 121, Issue 37, September 2024.
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PNAS:
Number: 37
Volume: 121
0 Replies | 222 Views
19F NMR as a Tool for Monitoring Individual Differentially Labeled Proteins in Complex Mixtures - American Chemical Society
Sep 10, 2024 - 4:30 PM - by nmrlearner
nmrlearner's Avatar 19F NMR as a Tool for Monitoring Individual Differentially Labeled Proteins in Complex Mixtures - American Chemical Society

19F NMR as a Tool for Monitoring Individual Differentially Labeled Proteins in Complex Mixtures American Chemical Society Read here
0 Replies | 13 Views
F-NMR of Nanofluorides: In Situ Quantification of Colloidal Diameters and Protein Corona Formation in Solution - American Chemical Society
Sep 10, 2024 - 4:30 PM - by nmrlearner
nmrlearner's Avatar F-NMR of Nanofluorides: In Situ Quantification of Colloidal Diameters and Protein Corona Formation in Solution - American Chemical Society

F-NMR of Nanofluorides: In Situ Quantification of Colloidal Diameters and Protein Corona Formation in Solution American Chemical Society Read here
0 Replies | 13 Views
Site-Specific Detection of Arginine Methylation in Highly Repetitive Protein Motifs of Low Sequence Complexity by NMR - American Chemical Society
Sep 10, 2024 - 4:30 PM - by nmrlearner
nmrlearner's Avatar Site-Specific Detection of Arginine Methylation in Highly Repetitive Protein Motifs of Low Sequence Complexity by NMR - American Chemical Society

Site-Specific Detection of Arginine Methylation in Highly Repetitive Protein Motifs of Low Sequence Complexity by NMR American Chemical Society Read here
0 Replies | 22 Views
AR conformational dynamics in a ternary complex with an engineered G protein viewed by NMR - ScienceDirect.com
Sep 10, 2024 - 4:30 PM - by nmrlearner
nmrlearner's Avatar AR conformational dynamics in a ternary complex with an engineered G protein viewed by NMR - ScienceDirect.com

AR conformational dynamics in a ternary complex with an engineered G protein viewed by NMR ScienceDirect.com Read here
0 Replies | 31 Views
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