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HybridNMR: A Union of Solution- and Solid-State NMR
Mar 24, 2017 - 10:00 AM - by nmrlearner
nmrlearner's Avatar HybridNMR: A Union of Solution- and Solid-State NMR

Pallavi Thiagarajan-Rosenkranz, Adrian W. Draney and Justin L. Lorieau



Journal of the American Chemical Society
DOI: 10.1021/jacs.6b11402




Source: Journal of the American Chemical Society
0 Replies | 5 Views
Heavy metal binding domain in a cysteine-rich protein may be sea snail adaptation to metal stress - Phys.Org
Mar 24, 2017 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Heavy metal binding domain in a cysteine-rich protein may be sea snail adaptation to metal stress - Phys.Org



Heavy metal binding domain in a cysteine-rich protein may be sea snail adaptation to metal stress
Phys.Org
Structural Adaptation of a Protein to Increased Metal Stress: NMR Structure of a Marine Snail Metallothionein with an Additional Domain. Credit: Wiley. A special type of small sulfur-rich protein, metallothioneins, have an extraordinary capability for ...


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0 Replies | 3 Views
Replication Protein A Blocks the Way
Mar 24, 2017 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Replication Protein A Blocks the Way



Biochemistry
DOI: 10.1021/acs.biochem.7b00221



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0 Replies | 4 Views
Protein Crystallization Market to Observe Strong Development by 2020 - Medgadget (blog)
Mar 23, 2017 - 8:51 PM - by nmrlearner
nmrlearner's Avatar Protein Crystallization Market to Observe Strong Development by 2020 - Medgadget (blog)


Medgadget (blog)


Protein Crystallization Market to Observe Strong Development by 2020
Medgadget (blog)
Various techniques used during protein crystallization technology are ion-exchange chromatography, high-performance liquid chromatography (HPLC), gel-electrophoresis, x-ray crystallography and nuclear magnetic resonance (NMR). On the basis of ...


... [Read More]
0 Replies | 7 Views
[NMR paper] Cu(II)-Based Paramagnetic Probe to Study RNA-Protein Interactions by NMR.
Mar 23, 2017 - 8:51 PM - by nmrlearner
nmrlearner's Avatar Cu(II)-Based Paramagnetic Probe to Study RNA-Protein Interactions by NMR.

Related Articles Cu(II)-Based Paramagnetic Probe to Study RNA-Protein Interactions by NMR.

Inorg Chem. 2017 Mar 22;

Authors: Seebald LM, DeMott CM, Ranganathan S, Asare Okai PN, Glazunova A, Chen A, Shekhtman A, Royzen M

Abstract
Paramagnetic NMR techniques allow for studying three-dimensional structures of RNA-protein complexes. In particular, paramagnetic relaxation enhancement (PRE) data can provide valuable information about long-range distances between different structural components. For PRE NMR experiments, oligonucleotides are typically spin-labeled using nitroxide reagents. The current work describes an alternative approach involving a Cu(II) cyclen-based probe that can be covalently attached to an RNA strand in the vicinity of the protein's binding site using "click" chemistry. The approach has been applied to study binding of HIV-1 nucleocapsid protein 7 (NCp7) to a model RNA pentanucleotide, 5'-ACGCU-3'. Coordination of the paramagnetic metal to glutamic acid residue of NCp7 reduced flexibility of the probe, thus simplifying interpretation of the PRE data. NMR experiments showed attenuation of signal intensities from protein residues localized in proximity to the paramagnetic probe as the result of RNA-protein interactions. The extent of the attenuation was related to the probe's proximity allowing us to... [Read More]
0 Replies | 10 Views
[NMR paper] Probing Medin Monomer Structure and its Amyloid Nucleation Using (13)C-Direct Detection NMR in Combination with Structural Bioinformatics.
Mar 23, 2017 - 8:51 PM - by nmrlearner
nmrlearner's Avatar Probing Medin Monomer Structure and its Amyloid Nucleation Using (13)C-Direct Detection NMR in Combination with Structural Bioinformatics.

Related Articles Probing Medin Monomer Structure and its Amyloid Nucleation Using (13)C-Direct Detection NMR in Combination with Structural Bioinformatics.

Sci Rep. 2017 Mar 22;7:45224

Authors: Davies HA, Rigden DJ, Phelan MM, Madine J

Abstract
Aortic medial amyloid is the most prevalent amyloid found to date, but remarkably little is known about it. It is characterised by aberrant deposition of a 5.4 kDa protein called medin within the medial layer of large arteries. Here we employ a combined approach of ab initio protein modelling and (13)C-direct detection NMR to generate a model for soluble monomeric medin comprising a stable core of three ?-strands and shorter more labile strands at the termini. Molecular dynamics simulations suggested that detachment of the short, C-terminal ?-strand from the soluble fold exposes key amyloidogenic regions as a potential site of nucleation enabling dimerisation and subsequent fibril formation. This mechanism... [Read More]
0 Replies | 10 Views
Interpolating and extrapolating with hmsIST:*seeking a t max for optimal sensitivity, resolution and frequency accuracy
Mar 23, 2017 - 8:30 AM - by nmrlearner
nmrlearner's Avatar Interpolating and extrapolating with hmsIST:*seeking a t max for optimal sensitivity, resolution and frequency accuracy

Abstract

Non-Uniform Sampling has the potential to exploit the optimal resolution of high-field NMR instruments. This is not possible in 3D and 4D NMR experiments when using traditional uniform sampling due to the long overall measurement time. Nominally, uniformly sampled time domain data acquired to a maximum evolution time tmax can be extended to high resolution via a virtual maximum evolution time t*max while extrapolating with linear prediction or iterative soft thresholding (IST). At the high resolution obtainable with extrapolation of US data, however, the accuracy of peak positions is compromised as observed when comparing inter- and intra-residue peaks in a 3D HNCA experiment. However, the accuracy of peak positions is largely improved by spreading the same number of acquired time domain data points non-uniformly over a larger evolution time to an optimal tmax followed by extrapolation to a total t*max and processing the data with an appropriate reconstruction method, such as hmsIST. To explore the optimum value of experimentally measured tmax to be reached non-uniformly with a given number of sampling points we have created test situations of time-equivalent experiments and evaluate sensitivity and accuracy of peak positions. Here we use signal-to-maximum-noise ratio as the decisive measure of sensitivity. We find that both sensitivity and resolution are optimal when PoissonGap sampling to a tmax of about ½*T2 *. Digital resolution is further enhanced by extrapolating the range of acquired time domain data to 2*T2 * but... [Read More]
0 Replies | 7 Views
Perspectives for hyperpolarisation in compact NMR
Mar 22, 2017 - 3:37 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Perspectives for hyperpolarisation in compact NMR

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Halse, M.E., Perspectives for hyperpolarisation in compact NMR. TrAC Trends in Analytical Chemistry, 2016. 83, Part A: p. 76-83.


http://dx.doi.org/10.1016/j.trac.2016.05.004


Nuclear magnetic resonance (NMR) is one of the most powerful analytical techniques currently available, with applications in fields ranging from synthetic chemistry to clinical diagnosis. Due to the size and cost of high-field spectrometers, NMR is generally considered to be ill-suited for industrial environments and field work. This conventional wisdom is currently being challenged through the development of NMR systems that are smaller, cheaper, more robust and portable. Despite remarkable progress in this area, potential applications are often limited by low sensitivity. Hyperpolarisation techniques have the potential to overcome this limitation and revolutionise the use of compact NMR. This review describes the state-of-the-art in NMR hyperpolarisation and presents promising examples of its application to compact NMR. Both the benefits and challenges associated with the different hyperpolarisation approaches are discussed and applications where these technologies have the potential to make a significant impact are highlighted.
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