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[ASAP] Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM
Apr 07, 2023 - 6:10 AM - by nmrlearner
nmrlearner's Avatar [ASAP] Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM

Yun Huang, Krishna D. Reddy, Clay Bracken, Biao Qiu, Wenhu Zhan, David Eliezer, and Olga Boudker



Journal of the American Chemical Society
DOI: 10.1021/jacs.3c01003



Source: Journal of the American Chemical Society
0 Replies | 279 Views
Nuclear magnetic resonance analysis of protein–DNA interactions | Journal of The Royal Society Interface - Royal Society
Apr 07, 2023 - 6:10 AM - by nmrlearner
nmrlearner's Avatar Nuclear magnetic resonance analysis of protein–DNA interactions | Journal of The Royal Society Interface Royal Society
Nuclear magnetic resonance analysis of protein–DNA interactions | Journal of The Royal Society Interface - Royal Society
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0 Replies | 251 Views
Using protein charge properties for challenging separations - European Pharmaceutical Review
Apr 07, 2023 - 6:10 AM - by nmrlearner
nmrlearner's Avatar Using protein charge properties for challenging separations - European Pharmaceutical Review

Using protein charge properties for challenging separations European Pharmaceutical Review Read here
0 Replies | 257 Views
[NMR paper] Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM
Apr 07, 2023 - 6:10 AM - by nmrlearner
nmrlearner's Avatar Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM

Limited chemical shift dispersion represents a significant barrier to studying multistate equilibria of large membrane proteins by ^(19)F NMR. We describe a novel monofluoroethyl ^(19)F probe that dramatically increases the chemical shift dispersion. The improved conformational sensitivity and line shape enable the detection of previously unresolved states in one-dimensional (1D) ^(19)F NMR spectra of a 134 kDa membrane transporter. Changes in the populations of these states in response to...

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0 Replies | 271 Views
Quantitative 1H Nuclear Magnetic Resonance (qNMR) of Aromatic Amino Acids for Protein Quantification - MDPI
Apr 06, 2023 - 5:43 PM - by nmrlearner
nmrlearner's Avatar Quantitative 1H Nuclear Magnetic Resonance (qNMR) of Aromatic Amino Acids for Protein Quantification MDPI
Quantitative 1H Nuclear Magnetic Resonance (qNMR) of Aromatic Amino Acids for Protein Quantification - MDPI
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0 Replies | 245 Views
J-Edited DIffusional Proton Nuclear Magnetic Resonance Spectroscopic Measurement of Glycoprotein and ... - American Chemical Society
Apr 06, 2023 - 5:43 PM - by nmrlearner
nmrlearner's Avatar J-Edited DIffusional Proton Nuclear Magnetic Resonance Spectroscopic Measurement of Glycoprotein and ... American Chemical Society
J-Edited DIffusional Proton Nuclear Magnetic Resonance Spectroscopic Measurement of Glycoprotein and ... - American Chemical Society
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0 Replies | 301 Views
[NMR paper] 1H, 15N, and 13C chemical shift backbone resonance NMR assignment of the accumulation-associated protein (Aap) lectin domain from Staphylococcus epidermidis
Apr 06, 2023 - 5:43 PM - by nmrlearner
nmrlearner's Avatar 1H, 15N, and 13C chemical shift backbone resonance NMR assignment of the accumulation-associated protein (Aap) lectin domain from Staphylococcus epidermidis

Staphylococcus epidermidis is the leading causative agent for hospital-acquired infections, especially device-related infections, due to its ability to form biofilms. The accumulation-associated protein (Aap) of S. epidermidis is primarily responsible for biofilm formation and consists of two domains, A and B. It was found that the A domain is responsible for the attachment to the abiotic/biotic surface, whereas the B domain is responsible for the accumulation of bacteria during biofilm...

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0 Replies | 265 Views
[NMR paper] Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR
Apr 06, 2023 - 5:43 PM - by nmrlearner
nmrlearner's Avatar Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR


Angewandte Chemie International Edition, Volume 62, Issue 17, April 17, 2023.

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0 Replies | 237 Views
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