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Investigating Protein–Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy - John Wiley
May 10, 2023 - 9:54 AM - by nmrlearner
nmrlearner's Avatar Investigating Protein–Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy John Wiley
Investigating Protein–Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy - John Wiley
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0 Replies | 124 Views
[NMR paper] Structural Mechanism of Soluble N-ethylmaleimide-Sensitive Factor Attachment Protein Receptor Complex Assembly in Lipid Bilayers Revealed by Solid-State NMR
May 10, 2023 - 9:54 AM - by nmrlearner
nmrlearner's Avatar Structural Mechanism of Soluble N-ethylmaleimide-Sensitive Factor Attachment Protein Receptor Complex Assembly in Lipid Bilayers Revealed by Solid-State NMR

Synaptic vesicle fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, including synaptobrevin-2 (Syb-2), syntaxin-1 (Syx-1), and SNAP-25. However, it remains controversial whether the formation of thoroughly contacted ?-helical bundle from the SNARE motifs to the end of the transmembrane domains (TMDs) is necessary for SNARE-mediated membrane fusion. In this study, we characterized the conformation of Syb-2 in different assembly states...

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0 Replies | 137 Views
[NMR paper] Solution NMR backbone assignment of the SASH1 SLy proteins associated disordered region (SPIDER)
May 10, 2023 - 9:54 AM - by nmrlearner
nmrlearner's Avatar Solution NMR backbone assignment of the SASH1 SLy proteins associated disordered region (SPIDER)

SASH1 is a scaffold protein with context-dependent biological functions in cell adhesion, tumor metastasis, lung development, and pigmentation. As a member of the SLy protein family, it contains the conserved SLY, SH3, and SAM domains. The 19 kDa SLY domain harbors over 70% of the SASH1 variants associated with pigmentation disorders. However, its solution structure or dynamics have not been investigated yet, and its exact position in the sequence is not clearly defined. Based on the...

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0 Replies | 127 Views
[NMR paper] Unfolding under pressure: an NMR perspective
May 10, 2023 - 9:54 AM - by nmrlearner
nmrlearner's Avatar Unfolding under pressure: an NMR perspective

This review aims at analysing the role of solution nuclear magnetic resonance (NMR) in pressure-induced in vitro studies of protein unfolding. Although this transition has for many years been neglected because of technical difficulties, it provides important information about the forces that keep together protein structure. We first analyse what is pressure unfolding. We then provide a critical overview of how NMR has contributed to the field and evaluate the observables used in these studies....

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0 Replies | 134 Views
[ASAP] Profiling Enzyme Activity of l-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance
May 09, 2023 - 12:35 PM - by nmrlearner
nmrlearner's Avatar [ASAP] Profiling Enzyme Activity of l-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance

Rachayita Nag, Srishti Joshi, Anurag Singh Rathore, and Subhabrata Majumder



Journal of the American Chemical Society
DOI: 10.1021/jacs.3c02154



Source: Journal of the American Chemical Society
0 Replies | 207 Views
Protein-DNA Docking in Drug Discovery: Identifying Small ... - PharmiWeb.com
May 09, 2023 - 12:35 PM - by nmrlearner
nmrlearner's Avatar Protein-DNA Docking in Drug Discovery: Identifying Small ... - PharmiWeb.com

Protein-DNA Docking in Drug Discovery: Identifying Small ... PharmiWeb.com Read here
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High-Sensitivity Mid-IR Absorption Spectroscopy for Proteins in ... - Spectroscopy Online
May 09, 2023 - 12:35 PM - by nmrlearner
nmrlearner's Avatar High-Sensitivity Mid-IR Absorption Spectroscopy for Proteins in ... - Spectroscopy Online

High-Sensitivity Mid-IR Absorption Spectroscopy for Proteins in ... Spectroscopy Online Read here
0 Replies | 130 Views
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