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[NMR paper] Direct assignment of 13C solid-state NMR signals of TFoF1 ATP synthase subunit c-ring in lipid membranes and its implication for the ring structure.
Dec 05, 2017 - 7:35 PM - by nmrlearner
nmrlearner's Avatar Direct assignment of 13C solid-state NMR signals of TFoF1 ATP synthase subunit c-ring in lipid membranes and its implication for the ring structure.

Direct assignment of 13C solid-state NMR signals of TFoF1 ATP synthase subunit c-ring in lipid membranes and its implication for the ring structure.

J Biomol NMR. 2017 Dec 02;:

Authors: Kang SJ, Todokoro Y, Bak S, Suzuki T, Yoshida M, Fujiwara T, Akutsu H

Abstract
FoF1-ATP synthase catalyzes ATP hydrolysis/synthesis coupled with a transmembrane H+ translocation in membranes. The Fo c-subunit ring plays a major role in this reaction. We have developed an assignment strategy for solid-state 13C NMR (ssNMR) signals of the Fo c-subunit ring of thermophilic Bacillus PS3 (TFo c-ring, 72 residues), carrying one of the basic folds of membrane proteins. In a ssNMR spectrum of uniformly 13C-labeled sample, the signal overlap has been a major bottleneck because most amino acid residues are hydrophobic. To overcome signal overlapping, we developed a method designated as COmplementary Sequential assignment with MInimum Labeling Ensemble (COSMILE). According to this method, we generated three kinds of reverse-labeled samples to suppress signal overlapping. To assign the carbon signals sequentially, two-dimensional C?(i+1)-C'C?(i) correlation and dipolar assisted rotational resonance (DARR) experiments were performed under magic-angle sample spinning. On the basis of inter- and intra-residue 13C-13C chemical shift correlations, 97% of C?, 97% of C? and 92% of C' signals were assigned directly from the spectra. Secondary structure analysis predicted a hairpin fold of two helices with a central loop. The effects... [Read More]
0 Replies | 15 Views
Scientists Find Brake for Protein Shell of HIV, Blocking Infection - AJMC.com Managed Markets Network
Dec 05, 2017 - 3:43 AM - by nmrlearner
nmrlearner's Avatar Scientists Find Brake for Protein Shell of HIV, Blocking Infection - AJMC.com Managed Markets Network



Scientists Find Brake for Protein Shell of HIV, Blocking Infection
AJMC.com Managed Markets Network
A cascade of events occurs as the HIV virus develops, affecting its structure and ability to infect. But in this case, protein building blocks get cut from a larger, master protein called Gag. By integrating state-of-the-art techniques, including solid ...


Read here
0 Replies | 18 Views
Researchers Put the Brakes on HIV's Ability to Infect - Technology Networks
Dec 04, 2017 - 3:35 PM - by nmrlearner
nmrlearner's Avatar

Researchers Put the Brakes on HIV's Ability to Infect
Technology Networks
She is an expert in nuclear magnetic resonance (NMR) spectroscopy, which helps scientists identify and pinpoint the location of every atom in a structure and how each atom moves. ??Viruses like HIV and their constituent protein and nucleic acid ...



Researchers Put the Brakes on HIV's Ability to Infect - Technology Networks
More...
0 Replies | 16 Views
Photo-induced radical polarization and liquid-state dynamic nuclear polarization with fullerene nitroxide derivatives
Dec 04, 2017 - 3:35 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Photo-induced radical polarization and liquid-state dynamic nuclear polarization with fullerene nitroxide derivatives

DNP without microwave radiation? A very interesting approach.




Liu, G., et al., Photo-induced radical polarization and liquid-state dynamic nuclear polarization with fullerene nitroxide derivatives. Phys. Chem. Chem. Phys., 2017.


http://dx.doi.org/10.1039/C7CP06073D


We report on radical polarization and optically-driven liquid DNP with nitroxide radicals functionalized by photoexcitable fullerene derivatives. Pulse laser excitation of the fullerene moiety leads to a transient nitroxide radical polarization that is one order of magnitude larger than at Boltzmann equilibrium. Life time of radical polarization increases with size of the fullerene derivative and correlates with the electronic spin lattice relaxation time T1e. Overhauser NMR signal enhancements of toluene solvent protons were observed under steady-state illumination, which replaced microwave irradiation.
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica} p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}

Go to The DNP-NMR Blog for... [Read More]
0 Replies | 27 Views
Researchers discover 'brake' that interferes with HIV's development into infectious agent - News-Medical.net
Dec 03, 2017 - 5:29 PM - by nmrlearner
nmrlearner's Avatar

Researchers discover 'brake' that interferes with HIV's development into infectious agent
News-Medical.net
She is an expert in nuclear magnetic resonance (NMR) spectroscopy, which helps scientists identify and pinpoint the location of every atom in a structure and how each atom moves. "Viruses like HIV and their constituent protein and nucleic acid ...

and more »

Researchers discover 'brake' that interferes with HIV's development into infectious agent - News-Medical.net
More...
0 Replies | 19 Views
Direct assignment of 13 C solid-state NMR signals of TF o F 1 ATP synthase subunit c -ring in lipid membranes and its implication for the ring structure
Dec 03, 2017 - 4:52 AM - by nmrlearner
nmrlearner's Avatar Direct assignment of 13 C solid-state NMR signals of TF o F 1 ATP synthase subunit c -ring in lipid membranes and its implication for the ring structure

Abstract

FoF1-ATP synthase catalyzes ATP hydrolysis/synthesis coupled with a transmembrane H+ translocation in membranes. The Fo c-subunit ring plays a major role in this reaction. We have developed an assignment strategy for solid-state 13C NMR (ssNMR) signals of the Fo c-subunit ring of thermophilic Bacillus PS3 (TFo c-ring, 72 residues), carrying one of the basic folds of membrane proteins. In a ssNMR spectrum of uniformly 13C-labeled sample, the signal overlap has been a major bottleneck because most amino acid residues are hydrophobic. To overcome signal overlapping, we developed a method designated as COmplementary Sequential assignment with MInimum Labeling Ensemble (COSMILE). According to this method, we generated three kinds of reverse-labeled samples to suppress signal overlapping. To assign the carbon signals sequentially, two-dimensional Cα(i+1)??C?Cα(i) correlation and dipolar assisted rotational resonance (DARR) experiments were performed under magic-angle sample spinning. On the basis of inter- and intra-residue 13C??13C chemical shift correlations, 97% of Cα, 97% of Cβ and 92% of C? signals were assigned directly from the spectra. Secondary structure analysis predicted a hairpin fold of two helices with a central loop. The effects of saturated and unsaturated phosphatidylcholines on TFo c-ring structure were examined. The DARR spectra at 15*ms mixing time are essentially similar to each other in... [Read More]
0 Replies | 21 Views
Discovery puts the brakes on HIV's ability to infect: Researchers find ... - Science Daily
Dec 03, 2017 - 4:52 AM - by nmrlearner
nmrlearner's Avatar
Science Daily


Discovery puts the brakes on HIV's ability to infect: Researchers find ...
Science Daily
Researchers have discovered a 'brake' that interferes with HIV's development into an infectious agent. This mechanism prevents the capsid -- the protein shell covering the virus -- from forming.

and more »

Discovery puts the brakes on HIV's ability to infect: Researchers find ... - Science Daily
... [Read More]
0 Replies | 18 Views
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