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Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative ... - Nature.com
Mar 31, 2024 - 11:00 AM - by nmrlearner
nmrlearner's Avatar Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative ... - Nature.com

Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative ... Nature.com Read here
0 Replies | 96 Views
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
Mar 30, 2024 - 9:30 PM - by nmrlearner
nmrlearner's Avatar Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression

Abstract

In NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high field-shifted resonance, which was assigned by mutational studies to the â??toggle switch tryptophanâ??. Residue-selective labeling thus enables site-specific investigations of this functionally important residue. The method described here is inexpensive, requires minimal genetic manipulation and can be expected to be applicable for yeast expression of GPCRs at large.



Source: Journal of Biomolecular NMR
0 Replies | 509 Views
All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy - Nature.com
Mar 30, 2024 - 9:22 AM - by nmrlearner
nmrlearner's Avatar All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy - Nature.com

All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy Nature.com Read here
0 Replies | 89 Views
Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy - Nature.com
Mar 30, 2024 - 9:22 AM - by nmrlearner
nmrlearner's Avatar Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy - Nature.com

Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy Nature.com Read here
0 Replies | 77 Views
Protein structure determination in living cells by in-cell NMR spectroscopy - Nature.com
Mar 30, 2024 - 9:22 AM - by nmrlearner
nmrlearner's Avatar Protein structure determination in living cells by in-cell NMR spectroscopy - Nature.com

Protein structure determination in living cells by in-cell NMR spectroscopy Nature.com Read here
0 Replies | 82 Views
Expanding the membrane-protein NMR toolkit - Nature.com
Mar 30, 2024 - 9:22 AM - by nmrlearner
nmrlearner's Avatar Expanding the membrane-protein NMR toolkit - Nature.com

Expanding the membrane-protein NMR toolkit Nature.com Read here
0 Replies | 94 Views
[NMR paper] Dimerization of the beta-Hairpin Membrane-Active Cationic Antimicrobial Peptide Capitellacin from Marine Polychaeta: An NMR Structural and Thermodynamic Study
Mar 29, 2024 - 7:50 PM - by nmrlearner
nmrlearner's Avatar Dimerization of the beta-Hairpin Membrane-Active Cationic Antimicrobial Peptide Capitellacin from Marine Polychaeta: An NMR Structural and Thermodynamic Study

Capitellacin is the ?-hairpin membrane-active cationic antimicrobial peptide from the marine polychaeta Capitella teleta. Capitellacin exhibits antibacterial activity, including against drug-resistant strains. To gain insight into the mechanism of capitellacin action, we investigated the structure of the peptide in the membrane-mimicking environment of dodecylphosphocholine (DPC) micelles using high-resolution NMR spectroscopy. In DPC solution, two structural forms of capitellacin were observed:...

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0 Replies | 175 Views
[NMR paper] Metabolomic response to acute resistance exercise in healthy older adults by 1H-NMR
Mar 29, 2024 - 7:43 AM - by nmrlearner
nmrlearner's Avatar Metabolomic response to acute resistance exercise in healthy older adults by 1H-NMR

CONCLUSIONS: This study demonstrates that while many exercise-responsive metabolites change similarly in young and older adults, several demonstrate age-dependent changes even in the absence of evidence of sarcopenia or frailty.

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0 Replies | 96 Views
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