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[NMR paper] Tapping into the native Pseudomonas Bacterial Biofilm Structure by High-Resolution 1D and 2D MAS solid-state NMR
Oct 24, 2023 - 7:00 PM - by nmrlearner
nmrlearner's Avatar Tapping into the native Pseudomonas Bacterial Biofilm Structure by High-Resolution 1D and 2D MAS solid-state NMR

We present a high-resolution 1D and 2D magic-angle spinning (MAS) solid-state NMR (ssNMR) study to characterize native Pseudomonas fluorescens colony biofilms at natural abundance without isotope-labelling. By using a high-resolution INEPT-based 2D ¹ H- ^(13) C ssNMR spectrum and thorough peak deconvolution approach at the 1D ssNMR spectra, approximately 80/134 (in 1D/2D) distinct biofilm chemical sites were identified. We compared CP and INEPT ^(13) C ssNMR spectra to different signals...

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MB&B Seminar: Dorothee Kern, "Evolution of Protein Dynamics and ... - Yale School of Medicine
Oct 24, 2023 - 12:28 AM - by nmrlearner
nmrlearner's Avatar MB&B Seminar: Dorothee Kern, "Evolution of Protein Dynamics and ... - Yale School of Medicine

MB&B Seminar: Dorothee Kern, "Evolution of Protein Dynamics and ... Yale School of Medicine Read here
0 Replies | 275 Views
A change in rigidity switches the function of protein condensates ... - Newswise
Oct 23, 2023 - 11:10 AM - by nmrlearner
nmrlearner's Avatar A change in rigidity switches the function of protein condensates ... Newswise
A change in rigidity switches the function of protein condensates ... - Newswise
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0 Replies | 255 Views
Intrinsically disordered regions in TRPV2 mediate protein-protein ... - Nature.com
Oct 23, 2023 - 11:10 AM - by nmrlearner
nmrlearner's Avatar Intrinsically disordered regions in TRPV2 mediate protein-protein ... - Nature.com

Intrinsically disordered regions in TRPV2 mediate protein-protein ... Nature.com Read here
0 Replies | 256 Views
Revealing structural secrets of a key cancer protein - The Ohio State University News
Oct 22, 2023 - 11:46 AM - by nmrlearner
nmrlearner's Avatar Revealing structural secrets of a key cancer protein - The Ohio State University News

Revealing structural secrets of a key cancer protein The Ohio State University News Read here
0 Replies | 262 Views
[NMR paper] Backbone NMR resonance assignments for the C terminal domain of the Streptococcus mutans adhesin P1
Oct 22, 2023 - 11:46 AM - by nmrlearner
nmrlearner's Avatar Backbone NMR resonance assignments for the C terminal domain of the Streptococcus mutans adhesin P1

Adhesin P1 (aka AgI/II) plays a pivotal role in mediating Streptococcus mutans attachment in the oral cavity, as well as in regulating biofilm development and maturation. P1's naturally occurring truncation product, Antigen II (AgII), adopts both soluble, monomeric and insoluble, amyloidogenic forms within the bacterial life cycle. Monomers are involved in important quaternary interactions that promote cell adhesion and the functional amyloid form promotes detachment of mature biofilms. The...

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0 Replies | 378 Views
[NMR paper] Assignment of the disordered, proline-rich N-terminal domain of the tumour suppressor p53 protein using 1HN and 1H?-detected NMR measurements
Oct 21, 2023 - 12:03 PM - by nmrlearner
nmrlearner's Avatar Assignment of the disordered, proline-rich N-terminal domain of the tumour suppressor p53 protein using 1HN and 1H?-detected NMR measurements

Protein p53 is mostly known for playing a key role in tumour suppression, and mutations in the p53 gene are amongst the most frequent genomic events accompanying oncogenic transformation. Continuous research is conducted to target disordered proteins/protein regions for cancer therapy, for which atomic level information is also necessary. The disordered N-terminal part of p53 contains the transactivation and the proline-rich domains-which besides being abundant in proline residues-contains...

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0 Replies | 171 Views
[NMR paper] Solution NMR assignments and structure for the dimeric kinesin neck domain
Oct 21, 2023 - 12:03 PM - by nmrlearner
nmrlearner's Avatar Solution NMR assignments and structure for the dimeric kinesin neck domain

Kinesin is a motor protein, comprised of two heavy and two light chains that transports cargo along the cytoskeletal microtubule filament network. The heavy chain has a neck domain connecting the ATPase motor head responsible for walking along microtubules, with the stalk and subsequent tail domains that bind cargo. The neck domain consists of a coiled coli homodimer with about five heptad repeats, preceded by a linker region that joins to the ATPase head. Here we report ¹H, ^(15)N, and ^(13)C...

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0 Replies | 194 Views
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