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Solid-state NMR structure determination of a membrane protein in E ... - Science
Nov 02, 2023 - 12:38 PM - by nmrlearner
nmrlearner's Avatar Solid-state NMR structure determination of a membrane protein in E ... - Science

Solid-state NMR structure determination of a membrane protein in E ... Science Read here
0 Replies | 170 Views
Revealing structural secrets of key cancer protein - Mirage News
Nov 02, 2023 - 12:38 PM - by nmrlearner
nmrlearner's Avatar Revealing structural secrets of key cancer protein Mirage News
Revealing structural secrets of key cancer protein - Mirage News
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0 Replies | 220 Views
Dimeric Transmembrane Structure of the SARS-CoV-2 E Protein ... - Nature.com
Nov 01, 2023 - 3:27 PM - by nmrlearner
nmrlearner's Avatar Dimeric Transmembrane Structure of the SARS-CoV-2 E Protein ... - Nature.com

Dimeric Transmembrane Structure of the SARS-CoV-2 E Protein ... Nature.com Read here
0 Replies | 211 Views
[NMR paper] B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles
Nov 01, 2023 - 3:27 PM - by nmrlearner
nmrlearner's Avatar B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles

Amphitropic proteins and peptides reversibly partition from solution to membrane, a key process that regulates their functions. Experimental approaches classically used to measure protein partitioning into lipid bilayers, such as fluorescence and circular dichroism, are hardly usable when the peptides or proteins do not exhibit significant polarity and/or conformational changes upon membrane binding. Here, we describe binding to lipid vesicles (B2LiVe), a simple, robust, and widely applicable...

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0 Replies | 204 Views
[NMR paper] A 19F-qNMR-Guided Mathematical Model for G Protein-Coupled Receptor Signaling
Nov 01, 2023 - 3:27 PM - by nmrlearner
nmrlearner's Avatar A 19F-qNMR-Guided Mathematical Model for G Protein-Coupled Receptor Signaling

GPCRs exhibit a wide range of pharmacological efficacies, yet the molecular mechanisms responsible for the differential efficacies in response to various ligands remain poorly understood. This lack of understanding has hindered the development of a solid foundation for establishing a mathematical model for signaling efficacy. However, recent progress has been made in delineating and quantifying receptor conformational states and associating function with these conformations. This progress has...

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0 Replies | 154 Views
[NMR paper] Exploring Sulfur Sites in Proteins via Triple-Resonance 1H-Detected 77Se NMR
Nov 01, 2023 - 2:23 AM - by nmrlearner
nmrlearner's Avatar Exploring Sulfur Sites in Proteins via Triple-Resonance 1H-Detected 77Se NMR

NMR spectroscopy has been applied to virtually all sites within proteins and biomolecules; however, the observation of sulfur sites remains very challenging. Recent studies have examined ^(77)Se as a replacement for sulfur and applied ^(77)Se NMR in both the solution and solid states. As a spin-1/2 nuclide, ^(77)Se is attractive as a probe of sulfur sites, and it has a very large chemical shift range (due to a large chemical shift anisotropy), which makes it potentially very sensitive to...

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0 Replies | 160 Views
[NMR paper] Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei
Oct 31, 2023 - 10:49 AM - by nmrlearner
nmrlearner's Avatar Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei

Laccases efficiently reduce dioxygen to water in an active site containing a tri-nuclear copper centre (TNC). The dynamics of the protein matrix is a determining factor in the efficiency in catalysis. To probe mobility, nuclear magnetic resonance (NMR) spectroscopy is highly suitable. However, several factors complicate the assignment of resonances to active site nuclei in laccases. The paramagnetic nature causes large shifts and line broadening. Furthermore, the presence of slow chemical...

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0 Replies | 120 Views
[NMR paper] The long-standing relationship between paramagnetic NMR and iron-sulfur proteins: the mitoNEET example. An old method for new stories or the other way around?
Oct 31, 2023 - 10:49 AM - by nmrlearner
nmrlearner's Avatar The long-standing relationship between paramagnetic NMR and iron-sulfur proteins: the mitoNEET example. An old method for new stories or the other way around?

Paramagnetic NMR spectroscopy and iron-sulfur (Fe-S) proteins have maintained a synergic relationship for decades. Indeed, the hyperfine shifts with their temperature dependencies and the relaxation rates of nuclei of cluster-bound residues have been extensively used as a fingerprint of the type and of the oxidation state of the Fe-S cluster within the protein frame. The identification of NMR signals from residues surrounding the metal cofactor is crucial for understanding the structure-function...

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0 Replies | 136 Views
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