BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 




Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


» Online Users: 9
0 members and 9 guests
No Members online
Most users ever online was 292, 07-25-2011 at 03:18 PM.
» Welcome!

Welcome, NMR world!


Our visitors map.


» Log in
User Name Not a member yet?
Register Now!
Password
PhaseSegregation in Cs-, Rb- and K-DopedMixed-Cation (MA)x(FA)1–xPbI3 Hybrid Perovskites from Solid-StateNMR
Sep 28, 2017 - 6:47 AM - by nmrlearner
nmrlearner's Avatar PhaseSegregation in Cs-, Rb- and K-DopedMixed-Cation (MA)x(FA)1–xPbI3 Hybrid Perovskites from Solid-StateNMR

Dominik J. Kubicki, Daniel Prochowicz, Albert Hofstetter, Shaik M. Zakeeruddin, Michael Gra?tzel and Lyndon Emsley



Journal of the American Chemical Society
DOI: 10.1021/jacs.7b07223




Source: Journal of the American Chemical Society
0 Replies | 19 Views
Protein Crystallization & Crystallography Market 2016 By Industry Size, Share & Key Drivers 2024 - Medgadget (blog)
Sep 27, 2017 - 4:26 PM - by nmrlearner
nmrlearner's Avatar Protein Crystallization & Crystallography Market 2016 By Industry Size, Share & Key Drivers 2024 - Medgadget (blog)


Medgadget (blog)


Protein Crystallization & Crystallography Market 2016 By Industry Size, Share & Key Drivers 2024
Medgadget (blog)
The market is divided by method employed and analysis of the protein crystallization and crystallography market are NMR spectroscopy, X-ray diffraction, Electron microscopy. The X-ray crystallography section is anticipated to be the largest market ...


... [Read More]
0 Replies | 41 Views
NMR secondary structure and interactions of recombinant human MOZART1 protein, a component of the gamma-tubulin complex
Sep 27, 2017 - 4:26 PM - by nmrlearner
nmrlearner's Avatar NMR secondary structure and interactions of recombinant human MOZART1 protein, a component of the gamma-tubulin complex

Abstract

Mitotic-spindle organizing protein associated with a ring of ?-tubulin 1 (MOZART1) is an 8.5 kDa protein linked to regulation of ?-tubulin ring complexes (?TuRCs), which are involved in nucleation of microtubules. Despite its small size, MOZART1 represents a challenging target for detailed characterization in vitro. We described herein a protocol for efficient production of recombinant human MOZART1 in Escherichia coli and assessed the properties of the purified protein using a combination of size exclusion chromatography coupled with multiangle light scattering (SEC-MALS), dynamic light scattering (DLS), and nuclear magnetic resonance (NMR) experiments. MOZART1 forms heterogeneous oligomers in solution. We identified optimal detergent and buffer conditions for recording well resolved NMR experiments allowing nearly full protein assignment and identification of three distinct alpha-helical structured regions. Finally, using NMR, we showed that MOZART1 interacts with the N-terminus (residues 1–250) of GCP3 (?-tubulin complex protein 3). Our data illustrate the capacity of MOZART1 to form oligomers, promoting multiple contacts with a subset of protein partners in the context of microtubule nucleation.




More...
0 Replies | 32 Views
[NMR paper] Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein
Sep 27, 2017 - 4:26 PM - by nmrlearner
nmrlearner's Avatar Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein


Mapping the interaction sites between membrane spanning proteins is a key challenge in structural biology. In this study a carbene footprinting approach is developed and applied to identify the interfacial sites of a trimeric, integral membrane protein, OmpF, solubilised in micelles. The diazirine-based footprinting probe is effectively sequestered by, and incorporated into, the micelles leading to efficient labelling of the membrane-spanning regions of the protein upon irradiation at 349 nm. Areas associated with protein-protein interactions between the trimer subunits remained unlabelled, thus revealing their location.

More...
0 Replies | 27 Views
Hyperpolarized 13 C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model
Sep 27, 2017 - 4:26 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Hyperpolarized 13 C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model

p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Baligand, C., et al., Hyperpolarized 13 C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model. NMR Biomed, 2017. 30(10): p. e3765-n/a.


https://www.ncbi.nlm.nih.gov/pubmed/28708304


Acute kidney injury (AKI) is a major risk factor for the development of chronic kidney disease (CKD). Persistent oxidative stress and mitochondrial dysfunction are implicated across diverse forms of AKI and in the transition to CKD. In this study, we applied hyperpolarized (HP) 13 C dehydroascorbate (DHA) and 13 C pyruvate magnetic resonance spectroscopy (MRS) to investigate the renal redox capacity and mitochondrial pyruvate dehydrogenase (PDH) activity, respectively, in a murine model of AKI at baseline and 7 days after unilateral ischemia reperfusion injury (IRI). Compared with the contralateral sham-operated kidneys, the kidneys subjected to IRI showed a significant decrease in the HP 13 C vitamin C/(vitamin C + DHA) ratio, consistent with a decrease in redox capacity. The kidneys subjected to IRI also showed a significant decrease in the HP 13 C bicarbonate/pyruvate ratio, consistent with impaired PDH activity. The IRI kidneys showed a significantly higher HP 13 C lactate/pyruvate ratio at day 7 compared with baseline, although the 13 C lactate/pyruvate ratio was... [Read More]
0 Replies | 31 Views
[NMR paper] Radiofrequency fields in MAS solid state NMR probes.
Sep 26, 2017 - 12:42 PM - by nmrlearner
nmrlearner's Avatar Radiofrequency fields in MAS solid state NMR probes.

Radiofrequency fields in MAS solid state NMR probes.

J Magn Reson. 2017 Sep 06;284:20-32

Authors: Tošner Z, Purea A, Struppe JO, Wegner S, Engelke F, Glaser SJ, Reif B

Abstract
We present a detailed analysis of the radiofrequency (RF) field over full volume of a rotor that is generated in a solenoid coil. On top of the usually considered static distribution of amplitudes along the coil axis we describe dynamic radial RF inhomogeneities induced by sample rotation. During magic angle spinning (MAS), the mechanical rotation of the sample about the magic angle, a spin packet travels through areas of different RF fields and experiences periodical modulations of both the RF amplitude and the phase. These modulations become particularly severe at the end regions of the coil where the relative RF amplitude varies up to ±25% and the RF phase changes within ±30°. Using extensive numerical simulations we demonstrate effects of RF inhomogeneity on pulse calibration and for the ramped CP experiment performed at a wide range of MAS rates. In addition, we review various methods to map RF fields using a B0 gradient along the sample (rotor axis) for imaging purposes. Under such a gradient, a nutation experiment provides directly the RF amplitude distribution, a cross polarization experiment images the correlation of the RF fields on the two channels according to the Hartmann-Hahn matching condition, while a spin-lock experiment allows to calibrate the RF amplitude employing the rotary resonance recoupling condition. Knowledge of the RF field distribution in a coil provides key to understand its effects... [Read More]
0 Replies | 31 Views
[NMR] Postdoc position on SSNMR at the University of Lille, France #DNPNMR
Sep 25, 2017 - 8:42 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

[NMR] Postdoc position on SSNMR at the University of Lille, France #DNPNMR

Fromt the Ampere Magnetic Resonance List






Please forward to potential candidates.


Project title: Development of high-field (DNP)-NMR methods to detect quadrupolar nuclei on catalytic surfaces


A two-year postdoc position in solid-state NMR spectroscopy of advanced materials is available at the University of Lille, Lille, France. It will start preferably in December 2017.


Project description: The development of improved heterogeneous catalysts can be undertaken in a rational way by a better
understanding of their structures. Solid-state NMR spectroscopy is very well suited to the study of heterogeneous catalysts because it can give information on the local structure. However, the lack of sensitivity and resolution poses limit for the characterization of surface sites, notably when they are occupied by quadrupolar nuclei (11B, 17O, 27Al, 67Zn, 95Mo...) exhibiting NMR signal broaden by large quadrupolar interaction. This project aims at developing and applying novel solid-state high-field (DNP)NMR methods to probe the local environment of quadrupolar nuclei. It will provide unique insights into the structure of the catalytic surfaces, which will be useful to improve their performances.


Host and research infrastructure: Lille is a vibrant and handsome city, imbued with a rich history, located in the center of northwestern Europe (only 30 min by high-speed trains from Brussels, 1h from Paris and 1h30 from London). Lille is one of France’s top student cities and the university of Lille is a leading center for... [Read More]
0 Replies | 38 Views
» Stats
Members: 3,202
Threads: 20,604
Posts: 20,990
Top Poster: nmrlearner (18,048)
Welcome to our newest member, bpadmanabhan
Powered by vBadvanced CMPS v3.2.2

All times are GMT. The time now is 09:48 AM.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
vBCredits II Deluxe v1.2.0 Copyright © 2010 DragonByte Technologies
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

Map