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[NMR paper] NMR-identification of the interaction between BRCA1 and the intrinsically disordered monomer of the Myc-associated factor X
Dec 01, 2023 - 6:23 PM - by nmrlearner
nmrlearner's Avatar NMR-identification of the interaction between BRCA1 and the intrinsically disordered monomer of the Myc-associated factor X

The breast cancer susceptibility 1 (BRCA1) protein plays a pivotal role in modulating the transcriptional activity of the vital intrinsically disordered transcription factor MYC. In this regard, mutations of BRCA1 and interruption of its regulatory activity are related to hereditary breast and ovarian cancer (HBOC). Interestingly, so far, MYC's main dimerization partner MAX (MYC-associated factor X) has not been found to bind BRCA1 despite a high sequence similarity between both oncoproteins....

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0 Replies | 169 Views
[NMR paper] Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies
Dec 01, 2023 - 6:23 PM - by nmrlearner
nmrlearner's Avatar Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies

The importance and fast growth of therapeutic monoclonal antibodies, both innovator and biosimilar products, have triggered the need for the development of characterization methods at high resolution such as nuclear magnetic resonance (NMR) spectroscopy. However, the full power of NMR spectroscopy cannot be unleashed without labelling the mAb of interest with NMR-active isotopes. Here, we present strategies using either Komagataella phaffii (Pichia pastoris) or Escherichia coli that can be...

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0 Replies | 122 Views
[NMR paper] Solid-state NMR chemical shift analysis for determining the conformation of ATP bound to Na,K-ATPase in its native membrane
Dec 01, 2023 - 6:11 AM - by nmrlearner
nmrlearner's Avatar Solid-state NMR chemical shift analysis for determining the conformation of ATP bound to Na,K-ATPase in its native membrane

Structures of membrane proteins determined by X-ray crystallography and, increasingly, by cryo-electron microscopy often fail to resolve the structural details of unstable or reactive small molecular ligands in their physiological sites. This work demonstrates that ^(13)C chemical shifts measured by magic-angle spinning (MAS) solid-state NMR (SSNMR) provide unique information on the conformation of a labile ligand in the physiological site of a functional protein in its native membrane, by...

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0 Replies | 149 Views
Researchers fix chirality of helical proteins - Mirage News
Nov 30, 2023 - 1:54 PM - by nmrlearner
nmrlearner's Avatar Researchers fix chirality of helical proteins - Mirage News

Researchers fix chirality of helical proteins Mirage News Read here
0 Replies | 144 Views
[NMR paper] Distinct activation mechanisms of ?-arrestin-1 revealed by 19F NMR spectroscopy
Nov 30, 2023 - 1:54 PM - by nmrlearner
nmrlearner's Avatar Distinct activation mechanisms of ?-arrestin-1 revealed by 19F NMR spectroscopy

?-Arrestins (?arrs) are functionally versatile proteins that play critical roles in the G-protein-coupled receptor (GPCR) signaling pathways. While it is well established that the phosphorylated receptor tail plays a central role in ?arr activation, emerging evidence highlights the contribution from membrane lipids. However, detailed molecular mechanisms of ?arr activation by different binding partners remain elusive. In this work, we present a comprehensive study of the structural changes in...

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0 Replies | 170 Views
[NMR paper] 1H NMR Using Metabolic Study in Body Fluids for Diagnosis of Cryptococcal Meningitis in Adults
Nov 30, 2023 - 1:54 PM - by nmrlearner
nmrlearner's Avatar 1H NMR Using Metabolic Study in Body Fluids for Diagnosis of Cryptococcal Meningitis in Adults

CONCLUSION: This is the first study to explore biomarkers in serum, urine, and CSF in addition to radiological features and clinical symptoms. Hence, a quick, non-invasive prognosis and diagnosis of cryptococcal meningitis in adults can be made using clinical and microbiological investigation, as well as metabolomic analysis of urine samples. This study shows that urine can be used as a biofluid to differentiate between Cryptococcus meningitis in adults. However, when compared to the negative...

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0 Replies | 121 Views
Researchers identify dynamic behavior of key SARS-CoV-2 accessory protein - Phys.org
Nov 29, 2023 - 8:18 PM - by nmrlearner
nmrlearner's Avatar Researchers identify dynamic behavior of key SARS-CoV-2 accessory protein Phys.org
Researchers identify dynamic behavior of key SARS-CoV-2 accessory protein - Phys.org
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0 Replies | 122 Views
Key Covid Accessory Protein's Dynamic Behavior Identified - Mirage News
Nov 29, 2023 - 7:54 AM - by nmrlearner
nmrlearner's Avatar Key Covid Accessory Protein's Dynamic Behavior Identified Mirage News
Key Covid Accessory Protein's Dynamic Behavior Identified - Mirage News
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0 Replies | 130 Views
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