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Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... - Business Wire
Apr 08, 2024 - 3:06 PM - by nmrlearner
nmrlearner's Avatar Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... - Business Wire

Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... Business Wire Read here
0 Replies | 59 Views
NMR Structure of the ?-Hemoglobin Stabilizing Protein: INSIGHTS INTO CONFORMATIONAL HETEROGENEITY AND ... - ScienceDirect.com
Apr 08, 2024 - 3:06 PM - by nmrlearner
nmrlearner's Avatar NMR Structure of the ?-Hemoglobin Stabilizing Protein: INSIGHTS INTO CONFORMATIONAL HETEROGENEITY AND ... - ScienceDirect.com

NMR Structure of the ?-Hemoglobin Stabilizing Protein: INSIGHTS INTO CONFORMATIONAL HETEROGENEITY AND ... ScienceDirect.com Read here
0 Replies | 63 Views
Application of LF-NMR to the characterization of camellia oil-loaded pickering emulsion fabricated by soy protein isolate - ScienceDirect.com
Apr 01, 2024 - 4:34 PM - by nmrlearner
nmrlearner's Avatar Application of LF-NMR to the characterization of camellia oil-loaded pickering emulsion fabricated by soy protein isolate - ScienceDirect.com

Application of LF-NMR to the characterization of camellia oil-loaded pickering emulsion fabricated by soy protein isolate ScienceDirect.com Read here
0 Replies | 95 Views
The NMR structure of the Orf63 lytic developmental protein from lambda bacteriophage | Scientific Reports - Nature.com
Mar 31, 2024 - 11:10 PM - by nmrlearner
nmrlearner's Avatar The NMR structure of the Orf63 lytic developmental protein from lambda bacteriophage | Scientific Reports - Nature.com

The NMR structure of the Orf63 lytic developmental protein from lambda bacteriophage | Scientific Reports Nature.com Read here
0 Replies | 75 Views
NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems - Phys.org
Mar 31, 2024 - 11:10 PM - by nmrlearner
nmrlearner's Avatar NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems - Phys.org

NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems Phys.org Read here
0 Replies | 78 Views
Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative ... - Nature.com
Mar 31, 2024 - 11:00 AM - by nmrlearner
nmrlearner's Avatar Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative ... - Nature.com

Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative ... Nature.com Read here
0 Replies | 87 Views
Cholesterol Interaction with the Trimeric HIV Fusion Protein gp41 in Lipid Bilayers Investigated by Solid-State NMR ... - ScienceDirect.com
Mar 31, 2024 - 11:00 AM - by nmrlearner
nmrlearner's Avatar Cholesterol Interaction with the Trimeric HIV Fusion Protein gp41 in Lipid Bilayers Investigated by Solid-State NMR ... - ScienceDirect.com

Cholesterol Interaction with the Trimeric HIV Fusion Protein gp41 in Lipid Bilayers Investigated by Solid-State NMR ... ScienceDirect.com Read here
0 Replies | 85 Views
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
Mar 30, 2024 - 9:30 PM - by nmrlearner
nmrlearner's Avatar Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression

Abstract

In NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high field-shifted resonance, which was assigned by mutational studies to the â??toggle switch tryptophanâ??. Residue-selective labeling thus enables site-specific investigations of this functionally important residue. The method described here is inexpensive, requires minimal genetic manipulation and can be expected to be applicable for yeast expression of GPCRs at large.



Source: Journal of Biomolecular NMR
0 Replies | 502 Views
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