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Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com

Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses Nature.com Read here
0 Replies | 88 Views
[NMR paper] Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning

Deuterium rotating frame solid-state NMR relaxation measurements (²H R(1?)) are important tools in quantitative studies of molecular dynamics. We demonstrate how ²H to ^(13)C cross-polarization (CP) approaches under 10-40 kHz magic angle spinning rates can be combined with the ²H R(1?) blocks to allow for extension of deuterium rotating frame relaxation studies to methyl groups in biomolecules. This extension permits detection on the ^(13)C nuclei and, hence, for the achievement of site-specific...

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0 Replies | 95 Views
[NMR paper] Interactions between the protein barnase and co-solutes studied by NMR
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Interactions between the protein barnase and co-solutes studied by NMR

Protein solubility and stability depend on the co-solutes present. There is little theoretical basis for selection of suitable co-solutes. Some guidance is provided by the Hofmeister series, an empirical ordering of anions according to their effect on solubility and stability; and by osmolytes, which are small organic molecules produced by cells to allow them to function in stressful environments. Here, NMR titrations of the protein barnase with Hofmeister anions and osmolytes are used to...

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0 Replies | 56 Views
[NMR paper] Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR

The coexistence of rigid and mobile molecules or molecular segments abounds in biomolecular assemblies. Examples include the carbohydrate-rich cell walls of plants and intrinsically disordered proteins that contain rigid ?-sheet cores. In solid-state nuclear magnetic resonance (NMR) spectroscopy, dipolar polarization transfer experiments are well suited for detecting rigid components, whereas scalar-coupling experiments are well suited for detecting highly mobile components. However, few NMR...

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0 Replies | 64 Views
Interactions between the protein barnase and co-solutes studied by NMR | Communications Chemistry - Nature.com
Feb 28, 2024 - 9:53 PM - by nmrlearner
nmrlearner's Avatar Interactions between the protein barnase and co-solutes studied by NMR | Communications Chemistry - Nature.com

Interactions between the protein barnase and co-solutes studied by NMR | Communications Chemistry Nature.com Read here
0 Replies | 68 Views
Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid - ScienceDirect.com
Feb 28, 2024 - 9:53 PM - by nmrlearner
nmrlearner's Avatar Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid - ScienceDirect.com

Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid ScienceDirect.com Read here
0 Replies | 77 Views
NMR Structure Determination for Larger Proteins Using Backbone-Only Data - Science
Feb 28, 2024 - 9:40 AM - by nmrlearner
nmrlearner's Avatar NMR Structure Determination for Larger Proteins Using Backbone-Only Data - Science

NMR Structure Determination for Larger Proteins Using Backbone-Only Data Science Read here
0 Replies | 84 Views
[NMR paper] XFEL structure of carbonic anhydrase II: a comparative study of XFEL, NMR, X-ray and neutron structures
Feb 27, 2024 - 9:30 PM - by nmrlearner
nmrlearner's Avatar XFEL structure of carbonic anhydrase II: a comparative study of XFEL, NMR, X-ray and neutron structures

The combination of X-ray free-electron lasers (XFELs) with serial femtosecond crystallography represents cutting-edge technology in structural biology, allowing the study of enzyme reactions and dynamics in real time through the generation of `molecular movies'. This technology combines short and precise high-energy X-ray exposure to a stream of protein microcrystals. Here, the XFEL structure of carbonic anhydrase II, a ubiquitous enzyme responsible for the interconversion of CO(2) and...

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0 Replies | 199 Views
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