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Antarctic bacterium has one of the biggest proteins ever found - Science Daily
Aug 11, 2017 - 8:52 PM - by nmrlearner
nmrlearner's Avatar

Antarctic bacterium has one of the biggest proteins ever found
Science Daily
"Apart from x-ray diffraction, we also used x-ray scattering, a specialist field of ours in Eindhoven, and nuclear magnetic resonance." Then they put all the pieces together like a jigsaw puzzle. Symbiosis. They discovered that each piece of the ...



Antarctic bacterium has one of the biggest proteins ever found - Science Daily
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[NMR] Training School on Principles and Applications of Dissolution DNP, Nov 13-17, 2017, Copenhagen
Aug 11, 2017 - 3:58 AM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

[NMR] Training School on Principles and Applications of Dissolution DNP, Nov 13-17, 2017, Copenhagen



Dear all,
The registration deadline for the training school, Principles and Applications of Dissolution DNP, has been extended to Sep 1.


The training school takes place at the Center for Hyperpolarization in Magnetic Resonance, at the Technical University of Denmark, Kgs Lyngby, Denmark from Nov 13-17, 2017.


We have three fantastic external lecturers signed up for the teaching, Tom Wenckebach, Matthew Merritt and Arnaud Comment, together with the faculty of the group. The school covers DNP theory, relaxation, kinetic modelling, sample preparation, polarizer instrumentation and operation, acquisition strategies as well as in vitro and in vivo applications. There will be plenty of hands-on exercises and in-depth discussion in small groups.


More details of the workshop is available from the website: http://www.hypermag.dtu.dk/Research/...ion-DNP-Course.


Please forward this email on to anyone who may be interested.


Thanks, and best regards, Jan


Jan Henrik Ardenkjaer-Larsen
Professor, Center Leader
Center for Magnetic Resonance
Technical University of Denmark
Department of Electrical Engineering
Ørsted Plads, bldg. 349, room 126
DK-2800 Kgs Lyngby
Phone +45 45253918
Email: ... [Read More]
0 Replies | 84 Views
[NMR paper] Solid-state NMR H-N-(C)-H and H-N-C-C 3D/4D correlation experiments for resonance assignment of large proteins.
Aug 10, 2017 - 1:27 PM - by nmrlearner
nmrlearner's Avatar Solid-state NMR H-N-(C)-H and H-N-C-C 3D/4D correlation experiments for resonance assignment of large proteins.

Related Articles Solid-state NMR H-N-(C)-H and H-N-C-C 3D/4D correlation experiments for resonance assignment of large proteins.

Chemphyschem. 2017 Aug 09;:

Authors: Fraga H, Arnaud CA, Gauto DF, Audin MJC, Kurauskas V, Macek P, Krichel C, Guan JY, Boisbouvier J, Sprangers R, Breyton C, Schanda P

Abstract
Solid-state NMR can provide insight into protein structure and dynamics at the atomic level without inherent protein size limitations. However, a major hurdle to studying large proteins by solid-state NMR spectroscopy is related to spectral complexity and resonance overlap, which increase with molecular weight and severely hamper the assignment process. Here we show the use of two sets of experiments that expand the tool kit of 1H-detected assignment approaches, and which correlate a given amide pair either to the two adjacent CO-CA pairs (4D hCOCANH/hCOCAcoNH), or to the amide 1H of the neighboring residue (3D HcocaNH/HcacoNH, which can be extended to up to 5D). The experiments are based on efficient coherence transfers between backbone atoms using INEPT transfers between carbons and cross-polarization for heteronuclear transfers. We exemplify the usefulness of these experiments with applications to assemblies of deuterated, fully amide-protonated proteins from ca. 20 to 60 kDa... [Read More]
0 Replies | 106 Views
[NMR paper] Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution*Measurements in Drug Products.
Aug 10, 2017 - 1:27 PM - by nmrlearner
nmrlearner's Avatar Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution*Measurements in Drug Products.

Related Articles Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution*Measurements in Drug Products.

AAPS J. 2017 Aug 08;:

Authors: Patil SM, Keire DA, Chen K

Abstract
Particle size distribution, a measurable physicochemical quantity, is a critical quality attribute of drug products that needs to be controlled in drug manufacturing. The non-invasive methods of dynamic light scattering (DLS) and Diffusion Ordered SpectroscopY (DOSY) NMR can be used to measure diffusion coefficient and derive the corresponding hydrodynamic radius. However, little is known about their use and sensitivity as analytical tools for particle size measurement of formulated protein therapeutics. Here, DLS and DOSY-NMR methods are shown to be orthogonal and yield identical diffusion coefficient results for a homogenous monomeric protein standard, ribonuclease A. However, different diffusion coefficients were observed for five insulin drug products measured using the two methods. DOSY-NMR yielded an averaged diffusion coefficient among fast exchanging insulin oligomers, ranging between dimer and hexamer*in size. By contrast, DLS showed several... [Read More]
0 Replies | 78 Views
[NMR paper] 1H NMR Metabolomics Reveals Association of High Expression of Inositol 1, 4, 5 Trisphosphate Receptor and Metabolites in Breast Cancer Patients.
Aug 10, 2017 - 12:13 AM - by nmrlearner
nmrlearner's Avatar 1H NMR Metabolomics Reveals Association of High Expression of Inositol 1, 4, 5 Trisphosphate Receptor and Metabolites in Breast Cancer Patients.

Related Articles 1H NMR Metabolomics Reveals Association of High Expression of Inositol 1, 4, 5 Trisphosphate Receptor and Metabolites in Breast Cancer Patients.

PLoS One. 2017;12(1):e0169330

Authors: Singh A, Sharma RK, Chagtoo M, Agarwal G, George N, Sinha N, Godbole MM

Abstract
1H NMR is used to detect alterations in metabolites and their linkage to metabolic processes in a number of pathological conditions including breast cancer. Inositol 1, 4, 5 trisphosphate (IP3R) receptor is an intracellular calcium channel known to regulate metabolism and cellular bioenergetics. Its expression is up regulated in a number of cancers. However, its linkage to metabolism in disease conditions has not... [Read More]
0 Replies | 37 Views
[NMR paper] A Pyrene-linked Cavity within a ?-Barrel Protein Promotes an Asymmetric Diels-Alder Reaction
Aug 10, 2017 - 12:13 AM - by nmrlearner
nmrlearner's Avatar A Pyrene-linked Cavity within a ?-Barrel Protein Promotes an Asymmetric Diels-Alder Reaction


A unique ?-expanded reaction cavity tethering a polycyclic moiety which provides a platform for substrate binding was constructed within the robust ?-barrel structure of nitrobindin (NB). NB variants with the cavities of different sizes and shapes are coupled with N-(1-pyrenyl)maleimide (Pyr) to prepare a series of NB-Pyr conjugates. The orientation of the pyrene moiety is fixed within the cavity by the coupling reaction. The fluorescent quenching analysis of NB-Pyr indicates that azachalcone (aza), which is a dienophile for a Diels-Alder (DA) reaction, is efficiently incorporated within the pyrene-linked reaction cavity by the aromatic interaction. The DA reaction between aza and cyclopentadiene proceeds within the reaction cavity of NB-Pyr in the presence Cu(II) ion in high yield and high enantio- and regioselectivity.

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0 Replies | 86 Views
Role of chiral quantum Hall edge states in nuclear spin polarization
Aug 10, 2017 - 12:13 AM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Role of chiral quantum Hall edge states in nuclear spin polarization

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Yang, K., et al., Role of chiral quantum Hall edge states in nuclear spin polarization. Nat Commun, 2017. 8: p. 15084.


https://www.ncbi.nlm.nih.gov/pubmed/28425462


Resistively detected NMR (RDNMR) based on dynamic nuclear polarization (DNP) in a quantum Hall ferromagnet (QHF) is a highly sensitive method for the discovery of fascinating quantum Hall phases; however, the mechanism of this DNP and, in particular, the role of quantum Hall edge states in it are unclear. Here we demonstrate the important but previously unrecognized effect of chiral edge modes on the nuclear spin polarization. A side-by-side comparison of the RDNMR signals from Hall bar and Corbino disk configurations allows us to distinguish the contributions of bulk and edge states to DNP in QHF. The unidirectional current flow along chiral edge states makes the polarization robust to thermal fluctuations at high temperatures and makes it possible to observe a reciprocity principle of the RDNMR response. These findings help us better understand complex NMR responses in QHF, which has important implications for the development of RDNMR techniques.
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