BioNMR

BioNMR (http://www.bionmr.com/forum/)
-   Solid-state high-res. NMR (http://www.bionmr.com/forum/solid-state-high-res-nmr-28/)
-   -   High Resolution 1H Detected 1H,13C Correlation Spectra in MAS Solid-State NMR using Deuterated Proteins with Selective 1H,2H Isotopic Labeling of Methyl Groups (http://www.bionmr.com/forum/solid-state-high-res-nmr-28/high-resolution-1h-detected-1h-13c-correlation-spectra-mas-solid-state-nmr-using-deuterated-proteins-selective-1h-2h-isotopic-labeling-methyl-groups-186/)

administrator 09-28-2006 05:42 AM

High Resolution 1H Detected 1H,13C Correlation Spectra in MAS Solid-State NMR using Deuterated Proteins with Selective 1H,2H Isotopic Labeling of Methyl Groups
 
2 Attachment(s)
High Resolution <SUP>1</SUP>H Detected <SUP>1</SUP>H,<SUP>13</SUP>C Correlation Spectra in MAS Solid-State NMR using Deuterated Proteins with Selective <SUP>1</SUP>H,<SUP>2</SUP>H Isotopic Labeling of Methyl Groups


Vipin Agarwal, Anne Diehl, Nikolai Skrynnikov, and Bernd Reif
J. Am. Chem. Soc.; 2006; 128(39) pp 12620 - 12621;

Abstract:
MAS solid-state NMR experiments applied to biological solids are still hampered by low sensitivity and resolution. In this work, we employ a deuteration scheme in which individual methyl groups are selectively protonated. This labeling scheme allows the acquisition of proton carbon correlation spectra with a resolution comparable to that in solution-state NMR experiments. We observe an increase in resolution by a factor of 10-15 compared to standard heteronuclear correlation experiments using PMLG for <SUP>1</SUP>H,<SUP>1</SUP>H dipolar decoupling in the indirect dimension. At the same time, the full sensitivity of the proton-based experiment is retained. In comparison to the heteronuclear detected version of the experiment, a gain in sensitivity of a factor of approximately 4.7 is achieved.

plazake 08-05-2009 03:21 AM

Wow! Been waiting for this! Thanks guys. Thanks Barry.
comparatif simulation taux credit auto - Taux crédit auto. Comparatif des offres! Les meilleurs taux crédit auto sont sur le net !comparatif simulation taux credit auto


All times are GMT. The time now is 03:48 PM.

Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2018, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013