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	[NMR paper] NMR metabolomic modeling of age and lifespan: A multicohort analysis NMR metabolomic modeling of age and lifespan: A multicohort analysis Metabolomic age models have been proposed for the study of biological aging, however, they have not been widely validated. We aimed to assess the performance of newly developed and existing nuclear magnetic resonance spectroscopy (NMR) metabolomic age models for prediction of chronological age (CA), mortality, and age-related disease. Ninety-eight metabolic variables were measured in blood from nine UK and Finnish cohort studies (N ?31,000 individuals, age range 24-86 years). We used nonlinear... More... nmrlearner	Today 05:58 AM by nmrlearner	0	9	Journal club
	[NMR paper] Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs ModA is a soluble periplasmic molybdate-binding protein found in most gram-negative bacteria. It is part of the ABC transporter complex ModABC that moves molybdenum into the cytoplasm, to be used by enzymes that carry out various redox reactions. Since there is no clear analog for ModA in humans, this protein could be a good target for antibacterial drug design. Backbone ¹H, ^(13)C and ^(15)N chemical shifts of apo and molybdate-bound ModA from E. coli were assigned at pHs 6.0 and 4.5. In... More... nmrlearner	Today 05:58 AM by nmrlearner	0	7	Journal club
	[NMR paper] Quantification of Residual Water in Pharmaceutical Frozen Solutions via (1)H Solid-state NMR Quantification of Residual Water in Pharmaceutical Frozen Solutions via (1)H Solid-state NMR Freezing is essential for the stability of biological drug substances and products, particularly in frozen solution formulations and during the primary drying of lyophilized preparations. However, the unfrozen segment within the frozen matrix can alter solute concentration, ionic strength, and stabilizer crystallization, posing risks of increased biophysical instability and faster chemical degradation. While quantifying the unfrozen water content is important for designing stable... More... nmrlearner	Today 05:58 AM by nmrlearner	0	6	Journal club
	[NMR paper] Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure Domain Z7 of nuclear transcription factor ZNF711 has the consensus last metal-ligand H23 found in odd-numbered zinc-fingers of this protein replaced by a phenylalanine. Ever since the discovery of ZNF711 it has been thought that Z7 is probably non-functional because of the H23F substitution. The presence of H26 three positions downstream prompted us to examine if this histidine could substitute as the last metal ligand. The Z7 domain adopts a stable tertiary structure... nmrlearner	Today 05:58 AM by nmrlearner	0	6	Journal club
	[NMR paper] Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI CONCLUSION: AAV2 viral capsids exhibit strong capacity as an endogenous CEST contrast agent and can potentially be used for monitoring and evaluation of AAV vector-mediated gene therapy protocols. More... nmrlearner	Today 05:58 AM by nmrlearner	0	7	Journal club
	[NMR paper] Protein Allostery Study in Cells Using NMR Spectroscopy Protein Allostery Study in Cells Using NMR Spectroscopy Protein allostery is commonly observed in vitro. But how protein allostery behaves in cells is unknown. In this work, a protein monomer-dimer equilibrium system was built with the allosteric effect on the binding characterized using NMR spectroscopy through mutations away from the dimer interface. A chemical shift linear fitting method was developed that enabled us to accurately determine the dissociation constant. A total of 28 allosteric mutations were prepared and grouped to negative... More... nmrlearner	Today 05:58 AM by nmrlearner	0	24	Journal club
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