BioNMR - Search Results

Showing results 1 to 4 of 4 Search took 0.00 seconds. Search: Threads Started By: Deano
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	ICMRBS founder’s medal 2006: Biological solid-state NMR, methods and applications ICMRBS founder’s medal 2006: Biological solid-state NMR, methods and applications Marc Baldus Journal of Biomolecular NMR; 2007; 39(1); pp 73-86 Abstract: Solid-state NMR (ssNMR) provides increasing possibilities to study structure and dynamics of biomolecular systems. Our group has been interested in developing ssNMR-based approaches that are applicable to biomolecules of increasing molecular size and complexity without the need of specific isotope-labelling. Methodological aspects ranging from spectral assignments to the indirect detection of proton–proton contacts in... Deano	08-14-2008 10:08 PM by Deano	0	3,176	Journal club
	Cell-free protein synthesis of perdeuterated proteins for NMR studies Cell-free protein synthesis of perdeuterated proteins for NMR studies Touraj Etezady-Esfarjani, Sebastian Hiller, Cristina Villalba and Kurt Wüthrich Journal of Biomolecular NMR; 2007; 39(3); pp 229-238 Abstract: Cell-free protein synthesis protocols for uniformly deuterated proteins typically yield low, non-uniform deuteration levels. This paper introduces an E. coli cell-extract, D-S30, which enables efficient production of proteins with high deuteration levels for all non-labile hydrogen atom positions. Potential applications of the new protocol may include production of proteins... Deano	08-14-2008 10:01 PM by Deano	0	3,905	Journal club
	CheckShift: automatic correction of inconsistent chemical shift referencing CheckShift: automatic correction of inconsistent chemical shift referencing Simon W. Ginzinger, Fabian Gerick, Murray Coles and Volker Heun Journal of Biomolecular NMR; 2007; 39(3); pp 223-227 Abstract: The construction of a consistent protein chemical shift database is an important step toward making more extensive use of this data in structural studies. Unfortunately, progress in this direction has been hampered by the quality of the available data, particularly with respect to chemical shift referencing, which is often either inaccurate or inconsistently annotated. Preprocessing of... Deano	08-14-2008 09:57 PM by Deano	0	3,312	Journal club
	Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments Mor Mishkovsky, Maayan Gal and Lucio Frydman Journal of Biomolecular NMR; 2007; 39(4); pp 291-301 Abstract: Three-dimensional nuclear magnetic resonance (3D NMR) provides one of the foremost analytical tools available for the elucidation of biomolecular structure, function and dynamics. Executing a 3D NMR experiment generally involves scanning a series of time-domain signals S(t 3), as a function of two time variables (t 1, t 2) which need to undergo parametric incrementations throughout... Deano	08-14-2008 09:53 PM by Deano	0	3,144	Journal club
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