Forum: NMR Questions and Answers
03-06-2013, 12:35 AM
|
Replies: 5
Views: 8,944
peptide NMR
Hi,
I did solved structure of 26 residue helical peptide and data was acquired on 700 MHz and also 500 MHz spectrometers (read this paper: Theoretical and in vitro studies of a C-terminal peptide...
|
Forum: Journal club
03-20-2012, 12:42 AM
|
Replies: 1
Views: 2,842
unlabeled protein NMR
Excellent to start using isotope reverse labeling for complete assignments in large/ unstructured proteins.
References:
Selective Isotopic Unlabeling of Proteins Using Metabolic Precursors:...
|
Forum: NMR Questions and Answers
11-15-2011, 12:00 PM
|
Replies: 2
Views: 5,524
alpha- alpha NOE
Hi,
If you are observing alpha-alpha NOEs continuously it must be beta sheet structure. In alpha helix you should observe continuous NH-NH NOEs. If both are present then your molecule must be...
|
Forum: NMR Questions and Answers
11-15-2011, 11:52 AM
|
Replies: 5
Views: 8,944
30 residue
Hi,
you can do NMR for 30 residue peptide on 500 MHz. Many people use 500 MHz for proteins itself. So you can proceed with 500 MHz. You need higher field only for better resolution and...
|
Forum: NMR Questions and Answers
11-15-2011, 11:46 AM
|
Replies: 2
Views: 5,494
cbcanh
Hi,
Changs the buffer to 50 mM sodium phosphate or sodium acatate and salt concentration to 50 mM NaCl or Kcl. You can try with 1 mM concentrated sample. And do HNCACB insted of CBCANH. It will...
|