BioNMR

BioNMR (http://www.bionmr.com/forum/)
-   Proteins (http://www.bionmr.com/forum/proteins-19/)
-   -   Optimisation of protein solubility for NMR (http://www.bionmr.com/forum/proteins-19/optimisation-protein-solubility-nmr-110/)

nmrlearner 08-14-2005 07:53 PM

A straight-forward method of optimising protein solubility for NMR.

Howe PW.

Analytical Sciences, Syngenta, Jealott's Hill Research Centre, Bracknell, Berkshire, RG42 6EY, UK.
http://public.metapress.com/images/c...ons/102922.jpg
J Biomol NMR. 2004 Nov;30(3):283-6

Abstract:
Maximising solubility is a key step in applying solution-state NMR to proteins. The 'microbatch' crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very small amounts of protein, is easy to set up and is readily automatable.


All times are GMT. The time now is 12:56 PM.

Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013