Protein aggregation Curling damage Nature.com
... misfolded proteins was induced. CsgC could inhibit CsgA amyloid formation in vitro, as detected by far-UV CD spectroscopy and NMR, presumably by stabilizing a preamyloid intermediate of CsgA, as detected by an amyloid conformation-specific antibody.
Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy
Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy
Abstract It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable species. The analysis was carried out using experimental data obtained during aggregation of the 10.4 kDa Crh protein, which has been shown to involve a partially unfolded intermediate...
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01-27-2011 04:31 AM
Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy.
Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy.
Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy.
J Biomol NMR. 2011 Jan 21;
Authors: Etzkorn M, Böckmann A, Baldus M
It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all...
nmrlearner
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01-22-2011 01:52 PM
[NMR paper] Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli. I
Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli. In vivo NMR studies.
Related Articles Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli. In vivo NMR studies.
J Biol Chem. 1998 Nov 13;273(46):30232-8
Authors: Dahan-Grobgeld E, Livneh Z, Maretzek AF, Polak-Charcon S, Eichenbaum Z, Degani H
Early metabolic events in Escherichia coli exposed to nalidixic acid, a topoisomerase II inhibitor and an inducer of the SOS system, were investigated by in vivo NMR spectroscopy, a technique...
nmrlearner
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11-17-2010 11:15 PM
[Question from NMRWiki Q&A forum] Why RF irradiation will damage amplifier?
Why RF irradiation will damage amplifier?
Here our 1H amplifier of NMR spectrometer is damaged twice for long time or high power RF irradiation. Why the duty cycle is important for amplifier or probe?If the damage happens to amplifier, which part of it is broken?
edit: The type of our spectrometer is Varian InfinityPlus for solids bought in 2003. I have no idea about the sort of amplifiers, just know the amplifier is for power output. Someone used about 80kHz 1H power for 1H decoupling during 100-200ms acquisition. The efficiency of the amplifier output droped gradually. The efficiency...
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News from other NMR forums
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09-28-2010 10:03 PM
NMR Metabolomic Profiling Reveals New Roles of SUMOylation in DNA Damage Response.
NMR Metabolomic Profiling Reveals New Roles of SUMOylation in DNA Damage Response.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles NMR Metabolomic Profiling Reveals New Roles of SUMOylation in DNA Damage Response.
J Proteome Res. 2010 Aug 9;
Authors: Cano KE, Li YJ, Chen Y
Post-translational modifications by the Small Ubiquitin-like Modifier (SUMO) family of proteins have been established as critical events in the cellular response to a wide range of DNA damaging reagents and radiation;...
Protein aggregation Curling damage - Nature.com
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