[NMR paper] NMRpQuant, an automated software for large scale urinary total protein quantification by one-dimensional 1H NMR profiles
NMRpQuant, an automated software for large scale urinary total protein quantification by one-dimensional 1H NMR profiles
SUMMARY: 1H nuclear magnetic resonance (NMR) spectroscopy is an established bioanalytical technology for metabolic profiling of biofluids in both clinical and large-scale population screening applications. Recently, urinary protein quantification has been demonstrated using the same 1D 1H NMR experimental data captured for metabolic profiling. Here we introduce NMRpQuant, a freely available platform that builds on these findings with both novel and further optimized...
[NMR paper] Cost-effective large-scale expression of proteins for NMR studies.
Cost-effective large-scale expression of proteins for NMR studies.
Related Articles Cost-effective large-scale expression of proteins for NMR studies.
J Biomol NMR. 2018 May 19;:
Authors: Klopp J, Winterhalter A, Gébleux R, Scherer-Becker D, Ostermeier C, Gossert AD
Abstract
We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and 13C-methyl methionine labeling in rich media, where yields of up to a gram of...
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05-21-2018 06:16 PM
Cost-effective large-scale expression of proteins for NMR studies
Cost-effective large-scale expression of proteins for NMR studies
Abstract
We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and 13C-methyl methionine labeling in rich media, where yields of up to a gram of soluble protein per liter of culture are reached. Procedures for uniform isotope labeling of 2H, 13C and 15N using auto-induction or isopropyl-β-d-1-thiogalactopyranoside-induction are described, with primary focus on...
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05-19-2018 05:41 PM
PhD Studentship – Applications of large-scale NMR, EPR and MRI simulations
From The DNP-NMR Blog:
PhD Studentship – Applications of large-scale NMR, EPR and MRI simulations
PhD Studentship – Applications of large-scale NMR, EPR and MRI simulations
PhD Supervisor: Ilya Kuprov
Application Deadline: 01 June 2016
Project Description:
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01-14-2016 07:24 AM
Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from ... - Nature.com
Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from ... - Nature.com
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Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from ...
Nature.com
The 1H and 13C NMR spectra (in acetone-d6) showed 23 proton and 16 carbon signals (Table 1) that were classified into one methyl, eight sp3 methylene, two sp3 oxygenated methine, two sp2 methine, two sp2 quaternary and one carbonyl carbons through ...
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04-22-2015 03:33 PM
[NMR paper] Application of sparse NMR restraints to large-scale protein structure prediction.
Application of sparse NMR restraints to large-scale protein structure prediction.
Related Articles Application of sparse NMR restraints to large-scale protein structure prediction.
Biophys J. 2004 Aug;87(2):1241-8
Authors: Li W, Zhang Y, Skolnick J
The protein structure prediction algorithm TOUCHSTONEX that uses sparse distance restraints derived from NMR nuclear Overhauser enhancement (NOE) data to predict protein structures at low-to-medium resolution was evaluated as follows: First, a representative benchmark set of the Protein Data Bank...
Halogenation of tyrosine perturbs large-scale protein self-organization - Nature.com
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