[NMR paper] Contribution of protein conformational heterogeneity to NMR lineshapes at cryogenic temperatures
Contribution of protein conformational heterogeneity to NMR lineshapes at cryogenic temperatures
While low temperature NMR holds great promise for the analysis of unstable samples and for sensitizing NMR detection, spectral broadening in frozen protein samples is a common experimental challenge. One hypothesis explaining the additional linewidth is that a variety of conformations are in rapid equilibrium at room temperature and become frozen, creating an inhomogeneous distribution at cryogenic temperatures. Here we investigate conformational heterogeneity by measuring the backbone...
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02-07-2023 04:23 PM
Characterization of conformational heterogeneity via higher-dimensionality, proton-detected solid-state NMR
Characterization of conformational heterogeneity via higher-dimensionality, proton-detected solid-state NMR
Abstract
Site-specific heterogeneity of solid protein samples can be exploited as valuable information to answer biological questions ranging from thermodynamic properties determining fibril formation to protein folding and conformational stability upon stress. In particular, for proteins of increasing molecular weight, however, site-resolved assessment without residue-specific labeling is challenging using established methodology, which tends...
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09-26-2022 02:08 AM
[NMR paper] Characterization of conformational heterogeneity via higher-dimensionality, proton-detected solid-state NMR
Characterization of conformational heterogeneity via higher-dimensionality, proton-detected solid-state NMR
Site-specific heterogeneity of solid protein samples can be exploited as valuable information to answer biological questions ranging from thermodynamic properties determining fibril formation to protein folding and conformational stability upon stress. In particular, for proteins of increasing molecular weight, however, site-resolved assessment without residue-specific labeling is challenging using established methodology, which tends to rely on carbon-detected 2D correlations. Here...
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09-26-2022 02:08 AM
[NMR paper] Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis.
Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis.
Related Articles Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis.
PeerJ. 2020;8:e9408
Authors: Wu S, Nguyen TTTN, Moroz OV, Turkenburg JP, Nielsen JE, Wilson KS, Rand KD, Teilum K
Abstract
Background: Several examples have emerged of enzymes where slow conformational changes are of key importance for function and where low populated conformations in the resting enzyme resemble the conformations of...
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07-04-2020 09:21 PM
Conformational heterogeneity in the Hsp70 chaperone-substrate ensemble identified from analysis of NMR-detected titration data
Conformational heterogeneity in the Hsp70 chaperone-substrate ensemble identified from analysis of NMR-detected titration data
Abstract
The Hsp70 chaperone system plays a critical role in cellular homeostasis by binding to client protein molecules. We have recently shown by methyl-TROSY NMR methods that the Escherichia coli Hsp70, DnaK, can form multiple bound complexes with a small client protein, hTRF1. In an effort to characterize the interactions further we report here the results of an NMR-based titration study of hTRF1 and DnaK, where both molecular components are monitored...
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09-18-2017 10:41 AM
[NMR paper] Information content of long-range NMR data for the characterization of conformational heterogeneity.
Information content of long-range NMR data for the characterization of conformational heterogeneity.
Related Articles Information content of long-range NMR data for the characterization of conformational heterogeneity.
J Biomol NMR. 2015 Jun 5;
Authors: Andra?oj? W, Berlin K, Fushman D, Luchinat C, Parigi G, Ravera E, Sgheri L
Abstract
Long-range NMR data, namely residual dipolar couplings (RDCs) from external alignment and paramagnetic data, are becoming increasingly popular for the characterization of conformational...
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06-06-2015 12:01 PM
Information content of long-range NMR data for the characterization of conformational heterogeneity
Information content of long-range NMR data for the characterization of conformational heterogeneity
Abstract
Long-range NMR data, namely residual dipolar couplings (RDCs) from external alignment and paramagnetic data, are becoming increasingly popular for the characterization of conformational heterogeneity of multidomain biomacromolecules and protein complexes. The question addressed here is how much information is contained in these averaged data. We have analyzed and compared the information content of conformationally averaged RDCs caused by...
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06-05-2015 01:10 AM
[NMR paper] Conformational heterogeneity of transmembrane residues after the Schiff base reproton
Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation of bacteriorhodopsin: 15N CPMAS NMR of D85N/T170C membranes.
Related Articles Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation of bacteriorhodopsin: 15N CPMAS NMR of D85N/T170C membranes.
FEBS J. 2005 May;272(9):2152-64
Authors: Mason AJ, Turner GJ, Glaubitz C
bR, N-like and O-like intermediate states of methionine-labelled wild type and D85N/T170C bacteriorhodopsin were accumulated in native membranes by...
Apolipoprotein E4 has extensive conformational heterogeneity in ... - pnas.org
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