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ppaudyal 02-15-2009 11:21 PM

Sequential Assignment of Spin systems
 
hi,
I am new to the protein NMR Field.I want to assign spin numbers to 120 amonoacid residues of a protein.I am cluless how to assing these number to each.Could anyone help me? Here is the primary sequence f a protein domain-

ISLLAQRQQF WIQLEFHSRI TRGERHGVID HVGLGVQSQQ RSNGFLTSLC ILRPHYASLS LALEKAQLHS LLCEETDGEG TLEYGFMGQV KSRFTDLSRP NLFCRHLGQL LPLLRVCRDV

I am expecing your help.Thank you.

Steve 03-13-2009 03:14 PM

Hi, you don't provide much information in your post - sequentially assigning a 120 amino acid protein can be quite a job.

Could you reply with what experiments you've run, and then we could try again?


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